Churchill A E
Vet Rec. 1987 Apr 4;120(14):334-9. doi: 10.1136/vr.120.14.334.
Canine parvovirus isolated from a case of haemorrhagic enteritis in a breeding kennel in England was passaged and cloned in cultured feline and canine cells. No significant evidence of pathogenicity was found during six serial passages of the modified virus back through young dogs. The attenuated virus was excreted by inoculated animals and spread rapidly to uninoculated animals held in contact. When high titre attenuated virus was given to the six-week-old offspring of a seropositive dam a prompt seroconversion was observed. When the attenuated virus was used as an experimental vaccine in 108 pups in an infected breeding colony a highly significant improvement was obtained in the accumulated morbidity and mortality compared with a parallel group vaccinated with modified live feline panleucopenia virus.
从英国一个繁殖犬舍的一例出血性肠炎病例中分离出的犬细小病毒，在培养的猫和犬细胞中传代并克隆。经修饰的病毒在幼犬中连续传代六次，未发现明显的致病性证据。接种动物排出了减毒病毒，并迅速传播到与之接触的未接种动物。当给血清阳性母犬六周龄的后代接种高滴度减毒病毒时，观察到迅速的血清转化。当减毒病毒在一个受感染的繁殖群体中的108只幼犬中用作实验性疫苗时，与接种改良活猫泛白细胞减少症病毒的平行组相比，累积发病率和死亡率有了显著改善。
