Department of Biochemistry, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada.
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S3E1, Canada.
Chembiochem. 2019 Feb 1;20(3):319-328. doi: 10.1002/cbic.201800538. Epub 2018 Dec 6.
Exploiting the innate modularity of proteins has allowed advances across the fields of synthetic biology and biotechnology. By using standardized protein components as building blocks, complex, multiprotein assemblies with sophisticated functions can be generated; feats previously not possible with strictly genetic-engineering approaches. The development of strategies for protein assembly is accelerating, pushing the boundaries of protein architecture. SpyTag and SpyCatcher protein ligase is a recent advance in this field that allows plug-and-play modularity by harnessing post-translational protein assembly. Herein, we review the latest applications of this powerful tool including novel enzyme assemblies, modularizing protein display, and the generation of antibody and antibody-like "devices" by using SpyTag/SpyCatcher technology.
利用蛋白质固有的模块性，可以在合成生物学和生物技术领域取得进展。通过使用标准化的蛋白质组件作为构建块，可以生成具有复杂功能的复杂多蛋白组装体；这是以前严格采用基因工程方法无法实现的壮举。蛋白质组装策略的发展正在加速，推动了蛋白质结构的边界。SpyTag 和 SpyCatcher 蛋白连接酶是该领域的一项最新进展，通过利用翻译后蛋白质组装实现了即插即用的模块化。本文综述了该强大工具的最新应用，包括新型酶组装体、模块化蛋白质展示以及使用 SpyTag/SpyCatcher 技术生成抗体和抗体样“器件”。
