Chapleau Richard R, Martin CharLee A, Hughes Summer R, Baldwin James C, Sladky John, Sherman Paul M, Grinkemeyer Michael
US Air Force School of Aerospace Medicine, Aeromedical Research Department, Applied Technology and Genomics Division, Wright-Patterson AFB, Dayton, OH, US.
US Air Force School of Aerospace Medicine, Aeromedical Research Department, Operational Health and Performance Research Division, Wright-Patterson AFB, Dayton, OH, US.
BMC Res Notes. 2018 Oct 25;11(1):764. doi: 10.1186/s13104-018-3867-7.
This study considers the use of a rapid molecular assay to evaluate apolipoprotein E (ApoE) status in military subjects who have been exposed to high altitude. We hypothesize that ApoE status may be protective against developing brain white matter hyperintensities (WMHs) after high altitude exposure.
We tested 92 subjects who had been exposed to altitudes above 25,000 ft mean sea level, either as pilots or as altitude chamber technicians. We determined subject genetic status using rapid Taqman-style polymerase chain reaction genotyping and evaluated the association of ApoE subtype versus brain lesions using t-tests and two-way analyses of variance. Our results indicate that there is no significant association between ApoE genotype status and the presence of WMHs after high altitude exposure. We did observe a significantly higher number of hours spent at altitude for subjects with the ApoE E2 allele; however, the mechanism by which this may occur is not determined in this study. To more fully elucidate this effect, larger populations would be required to observe greater numbers of subjects with the E2 and E4 alleles.
本研究探讨使用快速分子检测法评估暴露于高海拔地区的军事人员的载脂蛋白E（ApoE）状态。我们假设ApoE状态可能对高海拔暴露后发生脑白质高信号（WMH）具有保护作用。
我们检测了92名曾作为飞行员或高空气压舱技术人员暴露于平均海平面25,000英尺以上高度的受试者。我们使用快速Taqman式聚合酶链反应基因分型确定受试者的基因状态，并使用t检验和双向方差分析评估ApoE亚型与脑损伤之间的关联。我们的结果表明，高海拔暴露后ApoE基因型状态与WMH的存在之间没有显著关联。我们确实观察到携带ApoE E2等位基因的受试者在高海拔停留的时间明显更长；然而，本研究未确定这种情况可能发生的机制。为了更全面地阐明这种效应，需要更大的样本量来观察更多携带E2和E4等位基因的受试者。
