Department of Chemistry , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.
Department of Medicine , Imperial College London , London W12 0NN , U.K.
ACS Appl Mater Interfaces. 2018 Nov 7;10(44):38621-38629. doi: 10.1021/acsami.8b13721. Epub 2018 Oct 25.
Single-molecule methods have been rapidly developing with the appealing prospect of transforming conventional ensemble-averaged analytical techniques. However, challenges remain especially in improving detection sensitivity and controlling molecular transport. In this article, we present a direct method for the fabrication of analytical sensors that combine the advantages of nanopores and field-effect transistors for simultaneous label-free single-molecule detection and manipulation. We show that these hybrid sensors have perfectly aligned nanopores and field-effect transistor components making it possible to detect molecular events with up to near 100% synchronization. Furthermore, we show that the transport across the nanopore can be voltage-gated to switch on/off translocations in real time. Finally, surface functionalization of the gate electrode can also be used to fine tune transport properties enabling more active control over the translocation velocity and capture rates.
单分子方法发展迅速，有望改变传统的基于分子群体的分析技术。然而，仍然存在挑战，特别是在提高检测灵敏度和控制分子输运方面。在本文中，我们提出了一种直接的方法来制造分析传感器，该传感器结合了纳米孔和场效应晶体管的优势，用于同时进行无标记的单分子检测和操作。我们表明，这些混合传感器具有完美对齐的纳米孔和场效应晶体管组件，从而可以实现高达近 100%的同步检测分子事件。此外，我们表明，跨纳米孔的传输可以通过电压门控来实时开关转位。最后，栅极电极的表面功能化也可用于微调传输特性，从而能够更主动地控制转位速度和捕获率。
