Upcroft P
Aust J Exp Biol Med Sci. 1986 Dec;64 ( Pt 6):535-43. doi: 10.1038/icb.1986.58.
In extending the scope of our previous analyses of the functions that mammalian cells engender to recombine elements of genetic information (Upcroft, Carter and Kidson, 1980a and b), I describe the capacity of cells to recognise differentially combinations of open-ended and closed molecules. The frequency of successful recombination, as assayed by the generation of viable SV40 genomes from appropriate substrates, was shown to decrease with the loss of free termini capable of invasion of an homologous DNA duplex and to be influenced by the presence of non-homologous flanking sequences, the length of homology and whether the sequence was internal or terminal. The effect of stabilisation with long homologous regions on recombination over short homologous regions and inter-versus intra-molecular DNA sequence homologies was also investigated.
在扩展我们之前对哺乳动物细胞产生的重组遗传信息元件功能的分析范围时（Upcroft、Carter和Kidson，1980a和b），我描述了细胞识别开放式和封闭式分子不同组合的能力。通过从合适底物产生有活力的SV40基因组来测定成功重组的频率，结果表明，随着能够侵入同源DNA双链的自由末端的丧失，该频率会降低，并且受非同源侧翼序列的存在、同源性长度以及序列是内部的还是末端的影响。还研究了用长同源区域进行稳定化对短同源区域上的重组以及分子间与分子内DNA序列同源性的影响。
