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转染到哺乳动物细胞中的DNA分子间同源重组的拓扑学要求。

Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.

作者信息

Wake C T, Vernaleone F, Wilson J H

出版信息

Mol Cell Biol. 1985 Aug;5(8):2080-9. doi: 10.1128/mcb.5.8.2080-2089.1985.

DOI:10.1128/mcb.5.8.2080-2089.1985
PMID:3018550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366926/
Abstract

Cultured animal cells rearrange foreign DNA very efficiently by homologous recombination. The individual steps that constitute the mechanism(s) of homologous recombination in transfected DNA are as yet undefined. In this study, we examined the topological requirements by using the genome of simian virus 40 (SV40) as a probe. By assaying homologous recombination between defective SV40 genomes after transfection into CV1 monkey cells, we showed that linear molecules are preferred substrates for homologous exchanges, exchanges are distributed around the SV40 genome, and the frequency of exchange is not diminished significantly by the presence of short stretches of non-SV40 DNA at the ends. These observations are considered in relation to current models of homologous recombination in mammalian cells, and a new model is proposed. The function of somatic cell recombination is discussed.

摘要

培养的动物细胞通过同源重组非常有效地重排外源DNA。构成转染DNA中同源重组机制的各个步骤尚未明确。在本研究中，我们以猴病毒40（SV40）基因组为探针研究了拓扑学要求。通过检测缺陷型SV40基因组转染CV1猴细胞后的同源重组，我们发现线性分子是同源交换的首选底物，交换分布在SV40基因组周围，并且末端存在短片段非SV40 DNA时交换频率不会显著降低。结合当前哺乳动物细胞同源重组模型对这些观察结果进行了探讨，并提出了一个新模型。还讨论了体细胞重组的功能。

相似文献

1
Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.转染到哺乳动物细胞中的DNA分子间同源重组的拓扑学要求。
Mol Cell Biol. 1985 Aug;5(8):2080-9. doi: 10.1128/mcb.5.8.2080-2089.1985.
2
High frequency of homologous recombination in mammalian cells between endogenous and introduced SV40 genomes.哺乳动物细胞内源性与导入的SV40基因组之间同源重组的高频率。
Cell. 1985 Dec;43(3 Pt 2):695-703. doi: 10.1016/0092-8674(85)90242-9.
3
Quantitation of a simian virus 40 nonhomologous recombination pathway.猿猴病毒40非同源重组途径的定量分析。
J Virol. 1983 Oct;48(1):218-28. doi: 10.1128/JVI.48.1.218-228.1983.
4
How damaged is the biologically active subpopulation of transfected DNA?转染DNA的生物活性亚群受损程度如何？
Mol Cell Biol. 1984 Mar;4(3):387-98. doi: 10.1128/mcb.4.3.387-398.1984.
5
Modification of DNA ends can decrease end joining relative to homologous recombination in mammalian cells.在哺乳动物细胞中，DNA末端的修饰相对于同源重组而言，会降低末端连接。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4959-63. doi: 10.1073/pnas.84.14.4959.
6
Recombination between poly[d(GT).d(CA)] sequences in simian virus 40-infected cultured cells.猿猴病毒40感染的培养细胞中聚[d(GT).d(CA)]序列间的重组
Mol Cell Biol. 1985 Jun;5(6):1247-59. doi: 10.1128/mcb.5.6.1247-1259.1985.
7
Structure of simian virus 40-phiX174 recombinant genomes isolated from single cells.从单细胞中分离出的猿猴病毒40-φX174重组基因组的结构
J Virol. 1983 Oct;48(1):229-38. doi: 10.1128/JVI.48.1.229-238.1983.
8
Primary cells and established cell lines join DNA ends with the same efficiency relative to homologous recombination.原代细胞和已建立的细胞系在连接DNA末端方面，相对于同源重组具有相同的效率。
Plasmid. 1989 Sep;22(2):99-105. doi: 10.1016/0147-619x(89)90019-x.
9
Relative rates of homologous and nonhomologous recombination in transfected DNA.转染DNA中同源重组和非同源重组的相对速率。
Proc Natl Acad Sci U S A. 1985 May;82(10):3355-9. doi: 10.1073/pnas.82.10.3355.
10
Infection of eucaryotic cells by helper-independent recombinant adenoviruses: early region 1 is not obligatory for integration of viral DNA.辅助非依赖型重组腺病毒对真核细胞的感染：病毒DNA整合时早期区域1并非必需。
J Virol. 1984 May;50(2):606-14. doi: 10.1128/JVI.50.2.606-614.1984.

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A sensitive and rapid assay for homologous recombination in mosquito cells: impact of vector topology and implications for gene targeting.一种用于检测蚊细胞中同源重组的灵敏快速检测方法：载体拓扑结构的影响及对基因靶向的意义。
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Extrachromosomal recombination occurs efficiently in cells defective in various DNA repair systems.染色体外重组在各种DNA修复系统存在缺陷的细胞中能高效发生。
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A 5'-3' exonuclease from Saccharomyces cerevisiae is required for in vitro recombination between linear DNA molecules with overlapping homology.酿酒酵母的一种5'-3'核酸外切酶是线性DNA分子间具有重叠同源性的体外重组所必需的。
Mol Cell Biol. 1993 Jun;13(6):3125-34. doi: 10.1128/mcb.13.6.3125-3134.1993.

本文引用的文献

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The significance of responses of the genome to challenge.基因组对挑战做出反应的意义。
Science. 1984 Nov 16;226(4676):792-801. doi: 10.1126/science.15739260.
2
The recombination and integration of DNAs introduced into mouse L cells.导入小鼠L细胞的DNA的重组与整合。
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Recombination of transfected DNAs in vertebrate cells in culture.培养的脊椎动物细胞中转染DNA的重组。
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Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.合成寡核苷酸插入突变体在哺乳动物细胞同源重组研究中的新应用。
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5
High mutation frequency in DNA transfected into mammalian cells.转染到哺乳动物细胞中的DNA具有高突变频率。
Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9. doi: 10.1073/pnas.80.10.3015.
6
Recombination in adenovirus: analysis of crossover sites in intertypic overlap recombinants.腺病毒中的重组：不同血清型间重叠重组体中交叉位点的分析
Virology. 1984 Nov;139(1):43-52. doi: 10.1016/0042-6822(84)90328-3.
7
The double-strand-break repair model for recombination.用于重组的双链断裂修复模型。
Cell. 1983 May;33(1):25-35. doi: 10.1016/0092-8674(83)90331-8.
8
Conversion through homologous recombination of the gene encoding Simian virus 40 115,000-molecular-weight super T antigen to a gene encoding a normal-size large T antigen variant.通过同源重组将编码猿猴病毒40 115,000分子量超级T抗原的基因转化为编码正常大小大T抗原变体的基因。
Mol Cell Biol. 1984 Jun;4(6):1141-51. doi: 10.1128/mcb.4.6.1141-1151.1984.
9
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.DNA转入小鼠L细胞过程中的同源重组模型：DNA末端在重组过程中的作用。
Mol Cell Biol. 1984 Jun;4(6):1020-34. doi: 10.1128/mcb.4.6.1020-1034.1984.
10
Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.通过对输入DNA进行处理，可以增强哺乳动物细胞中质粒间的同源重组。
Proc Natl Acad Sci U S A. 1984 May;81(10):3153-7. doi: 10.1073/pnas.81.10.3153.