哺乳动物细胞中同源重组所需的最小同源性量。
The minimum amount of homology required for homologous recombination in mammalian cells.
作者信息
Rubnitz J, Subramani S
出版信息
Mol Cell Biol. 1984 Nov;4(11):2253-8. doi: 10.1128/mcb.4.11.2253-2258.1984.
Abstract
Although DNA sequence homology is believed to be a prerequisite for homologous recombination events in procaryotes and eucaryotes, no systematic study has been done on the minimum amount of homology required for homologous recombination in mammalian cells. We have used simian virus 40-pBR322 hybrid plasmids constructed in vitro as substrates to quantitate intramolecular homologous recombination in cultured monkey cells. Excision of wild-type simian virus 40 DNA by homologous recombination was scored by the viral plaque assay. Using a series of plasmids containing 0 to 243 base pairs of homology, we have shown that the recombination frequency decreases as the homology is reduced, with the sharpest drop in recombination frequency occurring when the homology was reduced from 214 to 163 base pairs. However, low recombination frequencies were also observed with as little as 14 base pairs of homology.
摘要
尽管DNA序列同源性被认为是原核生物和真核生物中同源重组事件的一个先决条件，但尚未对哺乳动物细胞中同源重组所需的最小同源量进行系统研究。我们使用体外构建的猴病毒40-pBR322杂交质粒作为底物，来定量培养的猴细胞中的分子内同源重组。通过病毒蚀斑测定法对同源重组导致的野生型猴病毒40 DNA的切除进行评分。使用一系列含有0至243个碱基对同源性的质粒，我们已经表明重组频率随着同源性的降低而降低，当同源性从214个碱基对减少到163个碱基对时，重组频率下降最为明显。然而，同源性低至14个碱基对时也观察到了低重组频率。
相似文献
1
The minimum amount of homology required for homologous recombination in mammalian cells.哺乳动物细胞中同源重组所需的最小同源性量。
Mol Cell Biol. 1984 Nov;4(11):2253-8. doi: 10.1128/mcb.4.11.2253-2258.1984.
2
Homologous and nonhomologous recombination in monkey cells.猴细胞中的同源和非同源重组
Mol Cell Biol. 1983 Jun;3(6):1040-52. doi: 10.1128/mcb.3.6.1040-1052.1983.
3
Rapid assay for extrachromosomal homologous recombination in monkey cells.猴细胞中染色体外同源重组的快速检测
Mol Cell Biol. 1985 Mar;5(3):529-37. doi: 10.1128/mcb.5.3.529-537.1985.
4
Somatic cells efficiently join unrelated DNA segments end-to-end.体细胞能有效地将不相关的DNA片段首尾相连。
Mol Cell Biol. 1982 Oct;2(10):1258-69. doi: 10.1128/mcb.2.10.1258-1269.1982.
5
Homologous recombination of monkey alpha-satellite repeats in an in vitro simian virus 40 replication system: possible association of recombination with DNA replication.猴α-卫星重复序列在体外猿猴病毒40复制系统中的同源重组：重组与DNA复制的可能关联
Mol Cell Biol. 1994 Jun;14(6):4173-82. doi: 10.1128/mcb.14.6.4173-4182.1994.
6
Mammalian cell functions mediating recombination of genetic elements: topological restraints.介导遗传元件重组的哺乳动物细胞功能：拓扑限制
Aust J Exp Biol Med Sci. 1986 Dec;64 ( Pt 6):535-43. doi: 10.1038/icb.1986.58.
7
Homologous recombination enhancement conferred by the Z-DNA motif d(TG)30 is abrogated by simian virus 40 T antigen binding to adjacent DNA sequences.由Z-DNA基序d(TG)30赋予的同源重组增强作用被猿猴病毒40 T抗原与相邻DNA序列的结合所消除。
Mol Cell Biol. 1990 Feb;10(2):794-800. doi: 10.1128/mcb.10.2.794-800.1990.
8
DNA sequence studies of simian virus 40 chromosomal excision and integration in rat cells.猴病毒40在大鼠细胞中染色体切除与整合的DNA序列研究。
J Mol Biol. 1984 Mar 25;174(1):55-84. doi: 10.1016/0022-2836(84)90365-6.
9
Analysis of recombination in mammalian cells using SV40 and SV40-derived vectors.使用SV40和SV40衍生载体分析哺乳动物细胞中的重组。
Mutat Res. 1989 Mar-May;220(2-3):221-34. doi: 10.1016/0165-1110(89)90026-2.
10
Homologous recombination in monkey cells and human cell-free extracts.猴细胞和人无细胞提取物中的同源重组
Cold Spring Harb Symp Quant Biol. 1984;49:191-7. doi: 10.1101/sqb.1984.049.01.022.
引用本文的文献
1
Mechanistic insights into 16p13.3 microdeletions encompassing TBC1D24 and ATP6V0C through advanced sequencing approaches.通过先进测序方法对包含TBC1D24和ATP6V0C的16p13.3微缺失的机制性见解。
Eur J Hum Genet. 2025 Jul 28. doi: 10.1038/s41431-025-01912-y.
2
Structural variation in 1,019 diverse humans based on long-read sequencing.基于长读长测序的1019名不同个体的结构变异
Nature. 2025 Jul 23. doi: 10.1038/s41586-025-09290-7.
3
Molecular mechanisms of extrachromosomal circular DNA formation.染色体外环状DNA形成的分子机制。
Nucleic Acids Res. 2025 Feb 27;53(5). doi: 10.1093/nar/gkaf122.
4
Genomic divergence across the tree of life.生命之树上的基因组差异。
Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2319389122. doi: 10.1073/pnas.2319389122. Epub 2025 Feb 27.
5
The Genome Sequences of Baculoviruses from the Tufted Apple Bud Moth, , Reveal Recombination Between an Alphabaculovirus and a Betabaculovirus from the Same Host.来自簇状苹果芽蛾的杆状病毒基因组序列揭示了同一宿主的甲型杆状病毒和乙型杆状病毒之间的重组。
Viruses. 2025 Jan 30;17(2):202. doi: 10.3390/v17020202.
6
Applications of advanced technologies for detecting genomic structural variation.先进技术在检测基因组结构变异中的应用。
Mutat Res Rev Mutat Res. 2023 Jul-Dec;792:108475. doi: 10.1016/j.mrrev.2023.108475. Epub 2023 Nov 4.
7
Easy efficient HDR-based targeted knock-in in genome using CRISPR-Cas9 system.利用 CRISPR-Cas9 系统在基因组中进行简便高效的 HDR 靶向基因敲入。
Bioengineered. 2022 Jun;13(6):14857-14871. doi: 10.1080/21655979.2022.2162667.
8
9
Generation of a Novel SARS-CoV-2 Sub-genomic RNA Due to the R203K/G204R Variant in Nucleocapsid: Homologous Recombination has Potential to Change SARS-CoV-2 at Both Protein and RNA Level.由于核衣壳中的R203K/G204R变异产生新型严重急性呼吸综合征冠状病毒2（SARS-CoV-2）亚基因组RNA：同源重组有可能在蛋白质和RNA水平上改变SARS-CoV-2 。
Pathog Immun. 2021 Aug 20;6(2):27-49. doi: 10.20411/pai.v6i2.460. eCollection 2021.
10
Nucleosome-induced homology recognition in chromatin.核小体诱导染色质中的同源识别。
J R Soc Interface. 2021 Jun;18(179):20210147. doi: 10.1098/rsif.2021.0147. Epub 2021 Jun 16.
本文引用的文献
1
Sister Chromatid Exchanges in Tritium-Labeled Chromosomes.氚标记染色体中的姐妹染色单体交换
Genetics. 1958 May;43(3):515-29. doi: 10.1093/genetics/43.3.515.
2
The kinetics of adenovirus recombination in homotypic and heterotypic genetic crosses.同型和异型基因杂交中腺病毒重组的动力学
Virology. 1980 Mar;101(2):503-15. doi: 10.1016/0042-6822(80)90464-x.
3
Inversions of specific DNA segments in flagellar phase variation of Salmonella and inversion systems of bacteriophages P1 and Mu.沙门氏菌鞭毛相变中特定DNA片段的倒位以及噬菌体P1和Mu的倒位系统
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7338-41. doi: 10.1073/pnas.77.12.7338.
4
P1 site-specific recombination: nucleotide sequence of the recombining sites.P1位点特异性重组：重组位点的核苷酸序列。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3398-402. doi: 10.1073/pnas.79.11.3398.
5
Analysis of the nucleotide sequence of an invertible controlling element.对一个可逆控制元件的核苷酸序列的分析。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4196-200. doi: 10.1073/pnas.77.7.4196.
6
Extrachromosomal circular copies of an 'inter-Alu' unstable sequence in human DNA are amplified during in vitro and in vivo ageing.人类DNA中“Alu序列间”不稳定序列的染色体外环状拷贝在体外和体内衰老过程中会扩增。
Nature. 1983 Feb 3;301(5899):394-8. doi: 10.1038/301394a0.
7
Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes.免疫球蛋白重链基因多样性DNA片段（D）的鉴定及核苷酸序列
Nature. 1981 Apr 16;290(5807):562-5. doi: 10.1038/290562a0.
8
By searching processively RecA protein pairs DNA molecules that share a limited stretch of homology.通过逐步搜索，RecA蛋白会配对具有有限同源片段的DNA分子。
Cell. 1983 Sep;34(2):647-54. doi: 10.1016/0092-8674(83)90397-5.
9
Evidence for intrachromosomal gene conversion in cultured mouse cells.培养的小鼠细胞中染色体内部基因转换的证据。
Cell. 1983 Nov;35(1):157-65. doi: 10.1016/0092-8674(83)90218-0.
10
Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.合成寡核苷酸插入突变体在哺乳动物细胞同源重组研究中的新应用。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4827-31. doi: 10.1073/pnas.80.15.4827.
Zotero 插件