Rubnitz J, Subramani S
Mol Cell Biol. 1984 Nov;4(11):2253-8. doi: 10.1128/mcb.4.11.2253-2258.1984.
Although DNA sequence homology is believed to be a prerequisite for homologous recombination events in procaryotes and eucaryotes, no systematic study has been done on the minimum amount of homology required for homologous recombination in mammalian cells. We have used simian virus 40-pBR322 hybrid plasmids constructed in vitro as substrates to quantitate intramolecular homologous recombination in cultured monkey cells. Excision of wild-type simian virus 40 DNA by homologous recombination was scored by the viral plaque assay. Using a series of plasmids containing 0 to 243 base pairs of homology, we have shown that the recombination frequency decreases as the homology is reduced, with the sharpest drop in recombination frequency occurring when the homology was reduced from 214 to 163 base pairs. However, low recombination frequencies were also observed with as little as 14 base pairs of homology.
尽管DNA序列同源性被认为是原核生物和真核生物中同源重组事件的一个先决条件，但尚未对哺乳动物细胞中同源重组所需的最小同源量进行系统研究。我们使用体外构建的猴病毒40-pBR322杂交质粒作为底物，来定量培养的猴细胞中的分子内同源重组。通过病毒蚀斑测定法对同源重组导致的野生型猴病毒40 DNA的切除进行评分。使用一系列含有0至243个碱基对同源性的质粒，我们已经表明重组频率随着同源性的降低而降低，当同源性从214个碱基对减少到163个碱基对时，重组频率下降最为明显。然而，同源性低至14个碱基对时也观察到了低重组频率。
