The Racah Institute of Physics, The Hebrew University, Jerusalem, 91904, Israel.
AMOLF Institute, Amsterdam, 1098XG, The Netherlands.
Nat Commun. 2018 Oct 25;9(1):4444. doi: 10.1038/s41467-018-06835-5.
The bacterial chemosensory arrays are a notable model for studying the basic principles of receptor clustering and cellular organization. Here, we provide a new perspective regarding the long-term dynamics of these clusters in growing E. coli cells. We demonstrate that pre-existing lateral clusters tend to avoid translocation to pole regions and, therefore, continually shuttle between the cell poles for many generations while being static relative to the local cell-wall matrix. We also show that the polar preference of clusters results fundamentally from reduced clustering efficiency in the lateral region, rather than a developmental-like progression of clusters. Furthermore, polar preference is surprisingly robust to structural alterations designed to probe preference due to curvature sorting, perturbing the cell envelope physiology affects the cluster-size distribution, and the size-dependent mobility of receptor complexes differs between polar and lateral regions. Thus, distinct envelope physiology in the polar and lateral cell regions may contribute to polar preference.
细菌的化学感觉阵列是研究受体聚类和细胞组织基本原理的重要模型。在这里，我们提供了关于这些在生长中的大肠杆菌细胞中进行长期动力学的新视角。我们证明了预先存在的侧部簇往往避免向极区转移，因此，在许多代中，它们在细胞两极之间持续穿梭，而相对于局部细胞壁基质保持静态。我们还表明，簇的极区偏好从根本上是由于在侧部区域的聚类效率降低所致，而不是类似于发育的簇的进行性发展。此外，极区偏好对旨在探测由于曲率分类而导致的偏好的结构改变非常稳健，干扰细胞包膜生理学影响簇大小分布，并且受体复合物的大小依赖性迁移率在极区和侧部区域之间有所不同。因此，极区和侧部细胞区域的不同包膜生理学可能有助于极区偏好。
