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植原体感染植物释放的挥发性有机化合物模式的收集、鉴定及统计分析

Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants.

作者信息

Gross Jürgen, Gallinger Jannicke, Rid Margit

机构信息

Laboratory of Applied Chemical Ecology, Institute for Plant Protection in Fruit Crops and Viticulture, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Dossenheim, Germany.

Plant Chemical Ecology, Technical University of Darmstadt, Darmstadt, Germany.

出版信息

Methods Mol Biol. 2019;1875:333-343. doi: 10.1007/978-1-4939-8837-2_25.

Abstract

In this chapter, we give an introduction to innovative attempts for the collection, identification, and statistical analysis of volatile organic compound (VOC) patterns emitted by phytoplasma-infected plants compared to healthy plants by the use of state-of-the-art techniques. This encompasses headspace-sampling techniques, gas chromatography coupled with mass spectrometry, and identification of VOC patterns by the "Automated Mass Spectral Deconvolution and Identification System" (AMDIS) followed by appropriate statistical analysis.

摘要

在本章中,我们将介绍一些创新性尝试,即运用先进技术,对受植原体感染的植物与健康植物所释放的挥发性有机化合物(VOC)模式进行收集、识别及统计分析。这包括顶空采样技术、气相色谱-质谱联用技术,以及通过“自动质谱解卷积与识别系统”(AMDIS)识别VOC模式,随后进行适当的统计分析。

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