Gross Jürgen, Gallinger Jannicke, Rid Margit
Laboratory of Applied Chemical Ecology, Institute for Plant Protection in Fruit Crops and Viticulture, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Dossenheim, Germany.
Plant Chemical Ecology, Technical University of Darmstadt, Darmstadt, Germany.
Methods Mol Biol. 2019;1875:333-343. doi: 10.1007/978-1-4939-8837-2_25.
In this chapter, we give an introduction to innovative attempts for the collection, identification, and statistical analysis of volatile organic compound (VOC) patterns emitted by phytoplasma-infected plants compared to healthy plants by the use of state-of-the-art techniques. This encompasses headspace-sampling techniques, gas chromatography coupled with mass spectrometry, and identification of VOC patterns by the "Automated Mass Spectral Deconvolution and Identification System" (AMDIS) followed by appropriate statistical analysis.
在本章中，我们将介绍一些创新性尝试，即运用先进技术，对受植原体感染的植物与健康植物所释放的挥发性有机化合物（VOC）模式进行收集、识别及统计分析。这包括顶空采样技术、气相色谱-质谱联用技术，以及通过“自动质谱解卷积与识别系统”（AMDIS）识别VOC模式，随后进行适当的统计分析。
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