Omega Optical, Brattleboro, Vermont.
Department of Pathology and Laboratory Medicine, University of Vermont Cancer Center, Burlington, Vermont.
J Cell Physiol. 2019 May;234(5):5413-5419. doi: 10.1002/jcp.27451. Epub 2018 Oct 26.
Novel optoelectronic instrumentation has been developed for the multispectral imaging of autofluorescence emitted by metabolic fluorophores. The images resolve individual cells while spectra are collected for each pixel in the images. These datacubes are generated at a rate of 10 per second-fast enough for surgical guidance. The data is processed in real time to provide a single color-coded image to the surgeon. To date, the system has been applied to fresh, ex vivo, human surgical specimens and has distinguished breast cancer from benign tissue. The approach is applicable to in vivo measurements of surgical margins and needle-based optical biopsies. Ongoing work demonstrates that the system has great potential for translation to a hand-held probe with high sensitivity and specificity.
已经开发出新型光电仪器，用于对代谢荧光团发出的自发荧光进行多光谱成像。这些图像可以分辨单个细胞，同时还可以为图像中的每个像素收集光谱。这些数据立方体的生成速度为每秒 10 个-足以用于手术指导。数据实时处理，为外科医生提供单个彩色编码图像。迄今为止，该系统已应用于新鲜的离体人手术标本，并将乳腺癌与良性组织区分开来。该方法适用于手术边缘和基于针的光学活检的体内测量。正在进行的工作表明，该系统具有很大的潜力，可以转化为具有高灵敏度和特异性的手持式探头。
