无标记荧光光谱法检测阿尔茨海默病小鼠模型脑组织中的关键生物分子
Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer's Disease.
机构信息
Institute for Ultrafast Spectroscopy and Lasers, the City College of the City University of New York, New York, NY, USA.
Department of Biology, the City College of the City University of New York, New York, NY, USA.
出版信息
Sci Rep. 2017 Jun 1;7(1):2599. doi: 10.1038/s41598-017-02673-5.
In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer's disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.
在这项研究中，我们首次使用无标记荧光光谱法测定了阿尔茨海默病（AD）小鼠模型新鲜脑组织中色氨酸、还原型烟酰胺腺嘌呤二核苷酸（NADH）和黄素腺嘌呤二核苷酸（FAD）的光谱特征。结果表明，AD 样本中色氨酸和 NADH 的发射光谱特征水平高于正常样本。AD 脑组织中色氨酸与 NADH 的强度比以及荧光强度随波长的变化率也有所增加。这些结果通过比较生物分子的光谱特征，为检测 AD 的早期阶段提供了一种光学方法。
Zotero 插件