Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia.
Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia; Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia.
J Chromatogr A. 2021 Feb 8;1638:461868. doi: 10.1016/j.chroma.2020.461868. Epub 2020 Dec 31.
One of the major drawbacks of electrophoresis in both capillary and microchip is the unsatisfactory sensitivity. Online sample preconcentration techniques can be regarded as the most common and powerful approaches commonly applied to enhance overall detection sensitivity. While the advances of various online preconcentration strategies in capillary and microchip employing aqueous background electrolytes are well-reviewed, there has been limited discussion of the feasible preconcentration techniques specifically developed for capillary and microchip using nonaqueous background electrolytes. This review provides the first consolidated overview of various online preconcentration techniques in nonaqueous capillary and microchip electrophoresis, covering the period of the last two decades. It covers developments in the field of sample stacking, isotachophoresis, and micellar-based stacking. Attention is also given to multi-stacking strategies that have been used for nonaqueous electrophoresis.
电泳在毛细管和微芯片中的主要缺点之一是灵敏度不理想。在线样品预浓缩技术可被视为最常用和最有效的方法，通常用于提高整体检测灵敏度。虽然已经对在使用水基背景电解质的毛细管和微芯片中采用的各种在线预浓缩策略进行了很好的综述，但对于专门为使用非水背景电解质的毛细管和微芯片开发的可行预浓缩技术的讨论却很有限。本综述首次对非水毛细管和微芯片电泳中的各种在线预浓缩技术进行了综合概述，涵盖了过去二十年的发展情况。它涵盖了样品堆积、等速电泳和胶束基堆积领域的发展。同时还关注了用于非水电泳的多堆积策略。
