一种用于肺再生的全自动高通量生物反应器系统。
A Fully Automated High-Throughput Bioreactor System for Lung Regeneration.
机构信息
1 Center for Regenerative Medicine , Massachusetts General Hospital, Boston, Massachusetts.
2 Harvard Medical School , Boston, Massachusetts.
出版信息
Tissue Eng Part C Methods. 2018 Nov;24(11):671-678. doi: 10.1089/ten.TEC.2018.0259.
Abstract
This work presents methods for ex vivo lung recellularization and biomimetic culture in a high-throughput and consistent manner. These methods allow for the testing of multiple variables, all of which are simultaneously controlled and monitored on a single fully automated pump system, and subsequent assessment of both epithelial and endothelial repair and tissue regeneration. This system provides a controlled environment for tissue repair, wherein key variables can be modified, monitored, reproduced, and optimized to advance the goal of ex vivo tissue regeneration based on native organ scaffolds.
摘要
本研究介绍了一种高通量且稳定的离体肺细胞再殖化和仿生培养方法。这些方法允许对多个变量进行测试，所有变量都在一个全自动泵系统上同时控制和监测，随后评估上皮细胞和内皮细胞的修复和组织再生。该系统为组织修复提供了一个受控环境，其中关键变量可以被修改、监测、复制和优化，以推进基于天然器官支架的离体组织再生目标。
相似文献
1
A Fully Automated High-Throughput Bioreactor System for Lung Regeneration.一种用于肺再生的全自动高通量生物反应器系统。
Tissue Eng Part C Methods. 2018 Nov;24(11):671-678. doi: 10.1089/ten.TEC.2018.0259.
2
Reconstructing the lung.重建肺部。
Science. 2010 Jul 30;329(5991):520-2. doi: 10.1126/science.1194087.
3
Ex vivo non-invasive assessment of cell viability and proliferation in bio-engineered whole organ constructs.生物工程全器官构建物中细胞活力和增殖的体外无创评估。
Biomaterials. 2015 Jun;52:103-12. doi: 10.1016/j.biomaterials.2015.01.061. Epub 2015 Feb 21.
4
Tissue-engineered lungs for in vivo implantation.用于体内植入的组织工程肺。
Science. 2010 Jul 30;329(5991):538-41. doi: 10.1126/science.1189345. Epub 2010 Jun 24.
5
Regenerative potential of human airway stem cells in lung epithelial engineering.人呼吸道干细胞在肺上皮工程中的再生潜力
Biomaterials. 2016 Nov;108:111-9. doi: 10.1016/j.biomaterials.2016.08.055. Epub 2016 Sep 4.
6
Re-endothelialization of rat lung scaffolds through passive, gravity-driven seeding of segment-specific pulmonary endothelial cells.通过特定肺段内皮细胞的被动重力驱动播种，实现大鼠肺支架的再内皮化。
J Tissue Eng Regen Med. 2018 Feb;12(2):e786-e806. doi: 10.1002/term.2382. Epub 2017 May 7.
7
Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration.用于高通量肺疾病和再生研究的去细胞化人肺和猪肺的三维支架。
Biomaterials. 2014 Mar;35(9):2664-79. doi: 10.1016/j.biomaterials.2013.11.078. Epub 2014 Jan 8.
8
Bioengineered lungs generated from human iPSCs-derived epithelial cells on native extracellular matrix.由人诱导多能干细胞衍生的上皮细胞在天然细胞外基质上生成的生物工程化肺。
J Tissue Eng Regen Med. 2018 Mar;12(3):e1623-e1635. doi: 10.1002/term.2589. Epub 2017 Nov 28.
9
Nonhuman primate lung decellularization and recellularization using a specialized large-organ bioreactor.使用专门的大型器官生物反应器对非人灵长类动物肺进行去细胞化和再细胞化。
J Vis Exp. 2013 Dec 15(82):e50825. doi: 10.3791/50825.
10
Use of High-Rate Ventilation Results in Enhanced Recellularization of Bioengineered Lung Scaffolds.高通气率的使用导致生物工程肺支架的再细胞化增强。
Tissue Eng Part C Methods. 2021 Dec;27(12):661-671. doi: 10.1089/ten.TEC.2021.0182.
引用本文的文献
1
Pre-vascularized porous gelatin-coated β-tricalcium phosphate scaffolds for bone regeneration: an in vivo and in vitro investigation.用于骨再生的预血管化多孔明胶包被β-磷酸三钙支架：一项体内和体外研究
In Vitro Cell Dev Biol Anim. 2025 Jan;61(1):67-80. doi: 10.1007/s11626-024-00973-5. Epub 2024 Oct 9.
2
Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering.去细胞支架中的血管生成与再内皮化：组织工程学的最新进展与当前挑战
Front Bioeng Biotechnol. 2023 Feb 16;11:1103727. doi: 10.3389/fbioe.2023.1103727. eCollection 2023.
3
Lung Tissue Engineering: Toward a More Deliberate Approach.肺组织工程：迈向更具策略性的方法。
ACS Biomater Sci Eng. 2022 Nov 14;8(11):4625-4628. doi: 10.1021/acsbiomaterials.1c01392. Epub 2022 Jan 24.
4
High-Throughput Culture Method of Induced Pluripotent Stem Cell-Derived Alveolar Epithelial Cells.高通量诱导多能干细胞衍生肺泡上皮细胞培养方法。
Tissue Eng Part C Methods. 2021 Dec;27(12):639-648. doi: 10.1089/ten.TEC.2021.0174.
5
Non-invasive and real-time measurement of microvascular barrier in intact lungs.无创实时测量完整肺的微血管屏障。
Biomaterials. 2019 Oct;217:119313. doi: 10.1016/j.biomaterials.2019.119313. Epub 2019 Jun 30.
本文引用的文献
1
Lung bioengineering: advances and challenges in lung decellularization and recellularization.肺生物工程：肺去细胞化和再细胞化的进展与挑战
Curr Opin Organ Transplant. 2018 Dec;23(6):673-678. doi: 10.1097/MOT.0000000000000584.
2
Extracellular matrix in lung development, homeostasis and disease.肺发育、稳态和疾病中的细胞外基质。
Matrix Biol. 2018 Nov;73:77-104. doi: 10.1016/j.matbio.2018.03.005. Epub 2018 Mar 8.
3
Bioengineering Human Lung Grafts on Porcine Matrix.生物工程猪基质上的人肺移植物。
Ann Surg. 2018 Mar;267(3):590-598. doi: 10.1097/SLA.0000000000002129.
4
Regenerative potential of human airway stem cells in lung epithelial engineering.人呼吸道干细胞在肺上皮工程中的再生潜力
Biomaterials. 2016 Nov;108:111-9. doi: 10.1016/j.biomaterials.2016.08.055. Epub 2016 Sep 4.
5
Bioengineering Lungs for Transplantation.用于移植的生物工程肺脏。
Thorac Surg Clin. 2016 May;26(2):163-71. doi: 10.1016/j.thorsurg.2015.12.004.
6
Engineering pulmonary vasculature in decellularized rat and human lungs.工程化去细胞化大鼠和人肺血管。
Nat Biotechnol. 2015 Oct;33(10):1097-102. doi: 10.1038/nbt.3354. Epub 2015 Sep 14.
7
A unique story in neonatal research: the development of a porcine surfactant.新生儿研究中的一个独特故事：猪肺表面活性剂的研发。
Neonatology. 2015;107(4):321-9. doi: 10.1159/000381117. Epub 2015 Jun 5.
8
Ex vivo non-invasive assessment of cell viability and proliferation in bio-engineered whole organ constructs.生物工程全器官构建物中细胞活力和增殖的体外无创评估。
Biomaterials. 2015 Jun;52:103-12. doi: 10.1016/j.biomaterials.2015.01.061. Epub 2015 Feb 21.
9
Design and validation of a clinical-scale bioreactor for long-term isolated lung culture.用于长期离体肺培养的临床规模生物反应器的设计与验证
Biomaterials. 2015 Jun;52:79-87. doi: 10.1016/j.biomaterials.2015.02.016. Epub 2015 Feb 23.
10
Extracellular matrix as a driver for lung regeneration.细胞外基质作为肺再生的驱动因素。
Ann Biomed Eng. 2015 Mar;43(3):568-76. doi: 10.1007/s10439-014-1167-5. Epub 2014 Oct 25.
Zotero 插件