Ueno Kotomi, Nakashima Hitomi, Mizutani Masaharu, Takikawa Hirosato, Sugimoto Yukihiro
Graduate School of Agricultural Science, Kobe University, Nada, Kobe 657-8501, Japan.
J Pestic Sci. 2018 Aug 20;43(3):198-206. doi: 10.1584/jpestics.D18-021.
The bioconversion of 5-deoxystrigol (5DS) and 4-deoxyorobanchol (4DO), the simplest canonical strigolactones (SLs), into monohydroxylated SLs such as strigol, sorgomol and orobanchol was confirmed by administering of stable isotope-labeled substrates to hydroponically grown plants. Liquid chromatography-mass spectrometry analyses established that 5DS was stereoselectively converted into strigol and sorgomol by cotton () and Chinese milk vetch (), respectively. 4DO was converted into orobanchol by rice (). However, the red bell pepper (), red clover (), and pea () negligibly converted 4DO into orobanchol. The red bell pepper converted -4DO into 2',8-bis-sorgomol. These results suggest that some plants generate orobanchol without passing through 4DO.
通过向水培植物施用稳定同位素标记的底物，证实了最简单的标准独脚金内酯（SLs）——5-脱氧独脚金醇（5DS）和4-脱氧列当醇（4DO）可生物转化为单羟基化的SLs，如独脚金醇、高独脚金醇和列当醇。液相色谱-质谱分析表明，5DS被棉花（）和紫云英（）分别立体选择性地转化为独脚金醇和高独脚金醇。4DO被水稻（）转化为列当醇。然而，红甜椒（）、红三叶草（）和豌豆（）将4DO转化为列当醇的量可忽略不计。红甜椒将-4DO转化为2'，8-双高独脚金醇。这些结果表明，一些植物无需通过4DO就能生成列当醇。
