Choupanian Choupanian Marziyeh Marziyeh, Omar Omar Dzolkhifli Dzolkhifli, Basri Basri Mahiran Mahiran, Asib Asib Norhayu Norhayu
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia.
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia.
J Pestic Sci. 2017 Nov 24;42(4):158-165. doi: 10.1584/jpestics.D17-032.
This study aimed to improve the efficacy of azadirachtin (. A. Juss) against two serious pest species of stored products, (L.) and (Herbst), through nano-emulsion formulations. Pseudoternary phase diagrams were constructed consisting of an emulsion system of an active ingredient (neem oil), surfactant (polysorbate or alkylpolyglucoside), and water. Isotropic regions were formed in the pseudoternary phase diagrams, and four formulations were selected from the isotropic regions and characterized according to particle size, particle aging, zeta potential, stability and thermostability, surface tension, viscosity, and pH. The selected formulations showed particle sizes of 208-507 nm in diameter. The result of contact toxicity demonstrated excellent mortality of and adults, with a mortality range of 85-100% and 74-100%, respectively, at a 1% azadirachtin concentration after only 2 days of exposure. Compared to non-formulated neem oil, the nano-emulsion formulations significantly increased the mortality of the tested species.
本研究旨在通过纳米乳液制剂提高印楝素（Azadirachtin (A. Juss)）对两种严重的仓储害虫——赤拟谷盗（Tribolium castaneum (Herbst)）和谷蠹（Rhyzopertha dominica (F.)）的防治效果。构建了由活性成分（印楝油）、表面活性剂（聚山梨酯或烷基多苷）和水组成的乳液体系的伪三元相图。在伪三元相图中形成了各向同性区域，从各向同性区域中选择了四种制剂，并根据粒径、颗粒老化、zeta电位、稳定性和热稳定性、表面张力、粘度和pH值进行了表征。所选制剂的粒径直径为208 - 507nm。接触毒性试验结果表明，仅在暴露2天后，在1%印楝素浓度下，赤拟谷盗和谷蠹成虫的死亡率极高，死亡率范围分别为85 - 100%和74 - 100%。与未配制的印楝油相比，纳米乳液制剂显著提高了受试物种的死亡率。
