Yousefi Mohammad-Reza, TaheriChadorneshin Hossein
Department of Sport Sciences, Ilam Branch, Islamic Azad University, Ilam, Islamic Republic of Iran.
Department of Sport Sciences, University of Bojnord, Bojnord, Islamic Republic of Iran.
Interv Med Appl Sci. 2018 Mar;10(1):59-63. doi: 10.1556/1646.9.2017.34.
Retinol-binding protein 4 (RBP4) is suggested to be involved in the occurrence of insulin resistance. There are contradictory studies about the effects of exercise training on RBP4 levels and insulin resistance. Hence, we designed this study to investigate the impact of moderate endurance training on gastrocnemius RBP4 and insulin resistance in streptozotocin (STZ)-induced diabetic rats.
Forty male albino Wistar rats were randomly divided into four groups: healthy control (HC), diabetic control (DC), healthy training (HT), and diabetic training (DT). Animals in HT and DT groups ran on a treadmill on the basis of overload principle for 6 weeks, three sessions per week. Rats in DC and DT groups are affected by diabetes using STZ (50 mg/kg of body weight). Gastrocnemius RBP4 content was measured using an enzyme-linked immunosorbent assay kit. Data were analyzed by one-way analysis of variance at < 0.05 level.
Serum blood glucose level ( = 0.001) and insulin resistance ( = 0.001) increased in DC compared with HC group, whereas serum insulin ( = 0.001) and gastrocnemius RBP4 ( = 0.001) reduced. However, there were no significant differences between serum blood glucose level ( = 0.384), insulin resistance ( = 0.999), and RBP4 ( = 0.999) content in DT compared with HT group.
Moderate endurance training reduces blood glucose level and subsequently improves insulin sensitivity by decreasing gastrocnemius RBP4 content independent of insulin.
视黄醇结合蛋白4（RBP4）被认为与胰岛素抵抗的发生有关。关于运动训练对RBP4水平和胰岛素抵抗的影响存在相互矛盾的研究。因此，我们设计了本研究，以探讨中等强度耐力训练对链脲佐菌素（STZ）诱导的糖尿病大鼠腓肠肌RBP4和胰岛素抵抗的影响。
40只雄性白化Wistar大鼠随机分为四组：健康对照组（HC）、糖尿病对照组（DC）、健康训练组（HT）和糖尿病训练组（DT）。HT组和DT组的动物根据超负荷原则在跑步机上跑步6周，每周3次。DC组和DT组的大鼠用STZ（50mg/kg体重）诱导糖尿病。使用酶联免疫吸附测定试剂盒测量腓肠肌RBP4含量。数据采用单因素方差分析，以<0.05为差异有统计学意义。
与HC组相比，DC组血清血糖水平（=0.001）和胰岛素抵抗（=0.001）升高，而血清胰岛素（=0.001）和腓肠肌RBP4（=0.001）降低。然而，与HT组相比，DT组血清血糖水平（=0.384）、胰岛素抵抗（=0.999）和RBP4（=0.999）含量无显著差异。
中等强度耐力训练可降低血糖水平，随后通过降低腓肠肌RBP4含量来提高胰岛素敏感性，且不依赖于胰岛素。
