Chalmers Gabhan, Davis Kristin E, Poljak Zvonimir, Friendship Robert, Mulvey Michael R, Deckert Anne E, Reid-Smith Richard J, Boerlin Patrick
Department of Pathobiology (Chalmers, Davis, Mulvey, Reid-Smith, Boerlin) and Department of Population Medicine, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1 (Poljak, Friendship, Deckert, Reid-Smith); National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba (Mulvey); Centre for Food-borne, Environmental Zoonotic Infectious Diseases, Public Health Agency of Canada, Guelph, Ontario (Deckert, Reid-Smith).
Can J Vet Res. 2018 Oct;82(4):312-315.
Colistin is one of the last-resort antibiotics for the treatment of multidrug-resistant infections in humans, but transmissible colistin-resistance genes have emerged in bacteria from animals. The rapid and sensitive detection among animals of colonization with bacteria carrying these genes is critical in helping to control further spread. Here we describe a method for broth enrichment of colistin-resistant from animal fecal and cecal samples followed by real-time polymerase chain reaction (PCR) for the simultaneous detection of two of the main colistin-resistance genes, and The PCR uses a single set of nondegenerative primers, and variants can be differentiated by melt-curve analysis. Overnight culture enrichment was effective for amplifying colistin-resistant even when initially present in numbers as low as 10 bacteria per gram of sample. The and genes were not found in any of the Ontario swine and poultry samples investigated.
黏菌素是治疗人类多重耐药感染的最后手段之一，但动物源细菌中已出现可传播的黏菌素耐药基因。在动物中快速、灵敏地检测携带这些基因的细菌定殖情况对于控制其进一步传播至关重要。在此，我们描述了一种从动物粪便和盲肠样本中富集耐黏菌素菌的肉汤培养方法，随后通过实时聚合酶链反应（PCR）同时检测两个主要的黏菌素耐药基因， 和 。该PCR使用一组非简并引物，通过熔解曲线分析可区分 变体。过夜培养富集对于扩增耐黏菌素菌很有效，即使最初每克样本中仅存在低至10个细菌。在所调查的安大略省猪和家禽样本中均未发现 和 基因。
