Corrêa Wallace Ribeiro, Serain Alessandra Freitas, Aranha Netto Leticia, Marinho Jane V N, Arena Arielle Cristina, Figueiredo de Santana Aquino Diana, Kuraoka-Oliveira Ângela Midori, Júnior Armando Jorge, Bernal Laura Priscila Toledo, Kassuya Cândida Aparecida Leite, Salvador Marcos José
Institute of Biology, Department of Plant Biology, PPG BTPB and PPGBV, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
Federal Institute of Education, Science and Technology, South of Minas Gerais (IFSULDEMINAS), 37576000 Inconfidentes, MG, Brazil.
Evid Based Complement Alternat Med. 2018 Sep 30;2018:6057579. doi: 10.1155/2018/6057579. eCollection 2018.
Brazilian ginseng, including , is used in popular medicine as a natural anti-inflammatory, tonic, analgesic, and antidiabetic agent. In this study, we investigated the chemical composition and evaluated the antioxidant and anti-inflammatory activities of the ethanolic extract as well as the major isolated glycoside flavonoids tiliroside and patuletin 3-O--D-glucopyranoside. Chromatographic techniques and spectroscopic analysis were used for the isolation and identification of the major compounds. The antioxidant potential was determined through DPPH and ORAC-FL assays. The total phenolic content was measured using Folin-Ciocalteu reagent. The anti-inflammatory activity was determined based on a model of paw edema and carrageenan- (Cg-) induced pleurisy. We identified three phenolic acids, one carboxylic acid and two flavonoids, patuletin 3-O--D-glucopyranoside, and tiliroside. The ethanol crude extracts, partitions and isolated flavonoids (4581 mol of Trolox equivalents/g of extract in ORAC and a SC of approximately 31.9 g/mL in the DPPH assay) demonstrated antioxidant activity, and the ethanolic extract as well as isolated flavonoids inhibited paw edema induced by Cg and leukocyte migration in the Cg-induced pleurisy model. The extract, tiliroside, and patuletin 3-O--D-glucopyranoside obtained from have therapeutic potential against oxidative stress-related and inflammatory disorders.
巴西人参，包括[具体种类未给出]，在传统医学中被用作天然抗炎、滋补、止痛和抗糖尿病药物。在本研究中，我们研究了[具体植物名称未给出]乙醇提取物以及主要分离出的糖苷类黄酮tiliroside和patuletin 3 - O - β - D - 吡喃葡萄糖苷的化学成分，并评估了其抗氧化和抗炎活性。采用色谱技术和光谱分析对主要化合物进行分离和鉴定。通过DPPH和ORAC - FL测定法测定抗氧化潜力。使用Folin - Ciocalteu试剂测量总酚含量。基于爪部水肿模型和角叉菜胶（Cg）诱导的胸膜炎模型测定抗炎活性。我们鉴定出三种酚酸、一种羧酸和两种黄酮类化合物，即patuletin 3 - O - β - D - 吡喃葡萄糖苷和tiliroside。乙醇粗提取物、各部分提取物和分离出的黄酮类化合物（在ORAC测定中为4581 μmol Trolox当量/克提取物，在DPPH测定中的半抑制浓度约为31.9 μg/mL）表现出抗氧化活性，并且乙醇提取物以及分离出的黄酮类化合物在Cg诱导的胸膜炎模型中抑制了Cg诱导的爪部水肿和白细胞迁移。从[具体植物名称未给出]中获得的提取物、tiliroside和patuletin 3 - O - β - D - 吡喃葡萄糖苷对氧化应激相关和炎症性疾病具有治疗潜力。
