Zhu Jiajie, Liu Shan, Guo Yu, Hou Liwei, Su Xiaolan, Li Yijie, Han Boyu, Liu Dengke, Wang Qingguo, Chen Jiande Jd, Wei Wei
Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
Department of Gastroenterology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China.
Evid Based Complement Alternat Med. 2018 Sep 30;2018:4280343. doi: 10.1155/2018/4280343. eCollection 2018.
The aim of this study was to examine a new method to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome.
A senna leaf (Folium sennae) decoction was made in 3 concentrations of 1.0, 0.5, and 0.25 g/mL. Rats were randomly divided into 4 groups: the control (C)-, high (H)-, middle (M)-, and low (L)- dose groups. The groups received saline, 1.0, 0.5, or 0.25 g/mL senna leaf decoction, respectively, for 4 weeks. Body weight monitoring, food consumption, water intake, defecation frequency, stool Bristol score, weight-loaded forced swimming test, forelimb grip strength test, D-xylose absorption test, serum cortisone, adrenocorticotropic hormone (ACTH), 24 h urine 17-hydroxycorticosteroid (17-OHCS), and histopathological detection were conducted to assess the success of the senna leaf decoction-induced model.
This study showed that the senna leaf decoction could induce diarrhea and dose-dependently slow body weight growth, reduce food consumption, and increase water intake, stool Bristol score, and defecation frequency. Statistical differences were found between groups H and M in rectal temperature, weight-loaded forced swimming time, forelimb grip strength, and serum cortisone. The D-xylose absorption test also showed dysfunction of intestinal absorption in groups H and M. The serum cortisone and 24 h urine 17-OHCS were significantly reduced in group H.
Gastric gavage of 10 mL/kg of body weight of a high concentration of a senna leaf decoction (1.0 g/mL) for 4 weeks was used to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome.
本研究旨在探讨一种建立脾肾阳虚证腹泻型大鼠模型的新方法。
制备三种浓度（1.0、0.5和0.25 g/mL）的番泻叶水煎剂。将大鼠随机分为4组：对照组（C）、高剂量组（H）、中剂量组（M）和低剂量组（L）。各组分别给予生理盐水、1.0、0.5或0.25 g/mL番泻叶水煎剂，连续4周。进行体重监测、食物摄入量、饮水量、排便频率、布里斯托大便评分、负重强迫游泳试验、前肢握力试验、D-木糖吸收试验、血清皮质酮、促肾上腺皮质激素（ACTH）、24小时尿17-羟皮质类固醇（17-OHCS）以及组织病理学检测，以评估番泻叶水煎剂诱导模型的成功与否。
本研究表明，番泻叶水煎剂可导致腹泻，并呈剂量依赖性地减缓体重增长、减少食物摄入量、增加饮水量、布里斯托大便评分和排便频率。H组和M组在直肠温度、负重强迫游泳时间、前肢握力和血清皮质酮方面存在统计学差异。D-木糖吸收试验也显示H组和M组存在肠道吸收功能障碍。H组血清皮质酮和24小时尿17-OHCS显著降低。
以10 mL/kg体重的高浓度（1.0 g/mL）番泻叶水煎剂灌胃4周，可建立脾肾阳虚证腹泻型大鼠模型。
