Ao Xiang, Zhang Zeying
Department of Pharmacy, Jiangxi College of Traditional Chinese Medicine, Fuzhou, China.
Food and Health Research Institute, Wuchang Institute of Technology, Wuhan, China.
Front Microbiol. 2024 Oct 15;15:1474374. doi: 10.3389/fmicb.2024.1474374. eCollection 2024.
To explore the effect of xylo-oligosaccharides on intestinal bacterial diversity in mice with spleen deficiency constipation.
The 16S rDNA sequencing was used to identify microbiota composition in four groups, including the normal group (NG), the model group with spleen-deficiency constipation (SDC), XOS treated groups that include XOS1 groups treated XOS 0.05 g/mL•d, and XOS2 group treated XOS 0.1 g/mL•d. Chao1 and Shannon were used to conduct gut microbes diversity analysis. Linear discriminant analysis coupled with effect size measurements (LEfSe) was used to identify signature gut microbiota, and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to predict the function of altered gut microbiota.
Veen map indicated 245 common OTUs were identified from four groups. Especially, 9, 3, 0, and 19 unique OTUs were identified in NG, SDC, XOS1, and XOS2 groups, respectively. The Shannon index was evidently higher in NG group than in the other three groups ( < 0.05). We identified the occurrence of dominant bacterial groups including Bacteroidetes (25.5 ~ 49.9%), Firmicutes (25.4 ~ 39.3%), Proteobacteria (12.5 ~ 24.9%), Deferribacteres (1.6 ~ 19.2%), Cyanobacteria (0.3 ~ 1.8%), Verrucomicrobia (0.02 ~ 1.6%), Actinobacteria (0.01 ~ 0.5%), and Tenericutes (0.03 ~ 0.09%) at the four groups. The XOS2 group was characterized by a higher abundance of , , , and . XOS1 group enriched in , , , , , , and . Meanwhile, the SDC mice showed dramatic enrichment in , , , , and , which were highly abundant in the NG group. XOS fed-mice evidently increase abundance compared with NG and SDC groups. However, the abundance of was significantly reduced in XOS1 and XOS2 groups compared with NG and SDC groups. We identified that altered gut microbiotas by XOS treatment were associated with various metabolic pathways, including organismal systems, metabolism, human diseases, genetic information processing, and cellular processes.
Our research indicated that XOS has the potential to recover intestinal bacteria and contribute to the treatment of spleen deficiency constipation.
探讨低聚木糖对脾虚便秘小鼠肠道细菌多样性的影响。
采用16S rDNA测序鉴定四组小鼠的微生物群组成，包括正常组（NG）、脾虚便秘模型组（SDC）、低聚木糖处理组，其中低聚木糖1组（XOS1组）给予0.05 g/mL•d的低聚木糖处理，低聚木糖2组（XOS2组）给予0.1 g/mL•d的低聚木糖处理。采用Chao1和Shannon指数进行肠道微生物多样性分析。利用线性判别分析结合效应大小测量（LEfSe）来识别标志性肠道微生物群，并通过重建未观察状态的群落系统发育研究（PICRUSt）来预测肠道微生物群改变后的功能。
Veen图显示四组共鉴定出245个常见操作分类单元（OTU）。特别是，在NG组、SDC组、XOS1组和XOS2组中分别鉴定出9个、3个、0个和19个独特的OTU。NG组的香农指数明显高于其他三组（P<0.05）。我们确定了四组中优势细菌群的存在，包括拟杆菌门（25.5%49.9%）、厚壁菌门（25.4%39.3%）、变形菌门（12.5%24.9%）、脱铁杆菌门（1.6%19.2%）、蓝细菌门（0.3%1.8%）、疣微菌门（0.02%1.6%）、放线菌门（0.01%0.5%）和柔膜菌门（0.03%0.09%）。XOS2组的特征是[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]和[具体细菌名称4]的丰度较高。XOS1组富含[具体细菌名称5]、[具体细菌名称6]、[具体细菌名称7]、[具体细菌名称8]、[具体细菌名称9]、[具体细菌名称10]和[具体细菌名称11]。同时，SDC小鼠的[具体细菌名称12]、[具体细菌名称13]、[具体细菌名称14]、[具体细菌名称15]和[具体细菌名称16]显著富集，而这些细菌在NG组中含量很高。与NG组和SDC组相比，低聚木糖喂养的小鼠[具体细菌名称17]丰度明显增加。然而，与NG组和SDC组相比，XOS1组和XOS2组中[具体细菌名称18]的丰度显著降低。我们发现低聚木糖处理改变的肠道微生物群与多种代谢途径有关，包括机体系统、代谢、人类疾病、遗传信息处理和细胞过程。
我们的研究表明，低聚木糖具有恢复肠道细菌的潜力，有助于脾虚便秘的治疗。
