National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Nucleic Acids Res. 2019 Jan 8;47(D1):D94-D99. doi: 10.1093/nar/gky989.
GenBank® (www.ncbi.nlm.nih.gov/genbank/) is a comprehensive database that contains publicly available nucleotide sequences for 420 000 formally described species. Most GenBank submissions are made using BankIt, the NCBI Submission Portal, or the tool tbl2asn, and are obtained from individual laboratories and batch submissions from large-scale sequencing projects, including whole genome shotgun (WGS) and environmental sampling projects. Daily data exchange with the European Nucleotide Archive (ENA) and the DNA Data Bank of Japan (DDBJ) ensures worldwide coverage. GenBank is accessible through the NCBI Nucleotide database, which links to related information such as taxonomy, genomes, protein sequences and structures, and biomedical journal literature in PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. Recent updates include an expansion of sequence identifier formats to accommodate expected database growth, submission wizards for ribosomal RNA, and the transfer of Expressed Sequence Tag (EST) and Genome Survey Sequence (GSS) data into the Nucleotide database.
GenBank®（www.ncbi.nlm.nih.gov/genbank/）是一个综合数据库，包含了 420000 个正式描述物种的公共核苷酸序列。大多数 GenBank 提交都是通过 BankIt、NCBI 提交门户或 tbl2asn 工具完成的，这些提交来自于各个实验室和大规模测序项目的批量提交，包括全基因组鸟枪法（WGS）和环境采样项目。与欧洲核苷酸档案馆（ENA）和日本 DNA 数据库（DDBJ）的每日数据交换确保了全球覆盖范围。GenBank 可通过 NCBI 核苷酸数据库访问，该数据库链接到相关信息，如分类学、基因组、蛋白质序列和结构，以及 PubMed 中的生物医学期刊文献。BLAST 提供了对 GenBank 和其他序列数据库的序列相似性搜索。GenBank 数据库的完整双月发布和每日更新可通过 FTP 获得。最近的更新包括扩展序列标识符格式以适应预期的数据库增长、核糖体 RNA 的提交向导，以及将表达序列标签（EST）和基因组调查序列（GSS）数据转移到核苷酸数据库。
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