Ramqvist T, Reinholdsson G, Szigeti R, Klein G, Dalianis T
Int J Cancer. 1987 Jul 15;40(1):74-80. doi: 10.1002/ijc.2910400114.
Two polyoma-TSTA-negative variants were selected independently from a polyoma fibrosarcoma/Moloney lymphoma somatic hybrid, by repeated passages in polyoma-virus-preimmunized mice. One of the variants had lost all its polyoma DNA, while the other only retained a deleted piece of its integrated polyoma DNA. In contrast to the parental hybrid clone, none of the variants produced detectable amounts of T-antigens. This finding indicates that a detectable expression of the products of the polyoma virus early genome, the T-antigens, is important either directly or indirectly for the expression of TSTA.
通过在预先用多瘤病毒免疫的小鼠中反复传代，从一个多瘤纤维肉瘤/莫洛尼淋巴瘤体细胞杂种中独立筛选出两个多瘤病毒肿瘤特异性移植抗原（TSTA）阴性变体。其中一个变体已失去其所有多瘤病毒DNA，而另一个仅保留了其整合的多瘤病毒DNA的一个缺失片段。与亲本杂种克隆不同，这些变体均未产生可检测量的T抗原。这一发现表明，多瘤病毒早期基因组产物T抗原的可检测表达，直接或间接地对于TSTA的表达很重要。
