胚胎细胞和多瘤病毒转化细胞的可溶性表面抗原的生物学活性
Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
作者信息
Kitahara Y, Barra Y, Meyer G
出版信息
Br J Cancer. 1978 Jan;37(1):41-7. doi: 10.1038/bjc.1978.6.
Various antigenic activities of polyoma virus-transformed and embryonic mouse cells were retained after 3M KCl solubilization of surface components. Particularly, transplantation antigen (TSTA) demonstrated by homograft rejection, and surface (S) antigen, detected by inhibition of immunofluorescence on polyoma-virus-transformed mouse cells, could be demonstrated. The crude soluble extracts were partially purified by salting out with (NH4)2SO4. In the case of polyoma-virus-transformed cells, TSTA and a part of S antigen activity were found in the same fraction (60% (NH4)2SO4 saturation) while another part of S antigen was salted out at 80% saturation. By chromatography, S antigen activity was found in 2 zones for transformed cells and in one zone for embryonic cells. One of these zones was common to both cell extracts.
在用3M KCl溶解表面成分后，多瘤病毒转化的小鼠细胞和胚胎小鼠细胞的各种抗原活性得以保留。特别是，通过同种异体移植排斥反应证明的移植抗原（TSTA）以及通过对多瘤病毒转化的小鼠细胞进行免疫荧光抑制检测到的表面（S）抗原都能够被证实。粗制的可溶性提取物通过用硫酸铵盐析进行部分纯化。对于多瘤病毒转化的细胞，TSTA和一部分S抗原活性存在于同一组分（60%硫酸铵饱和度）中，而另一部分S抗原在80%饱和度时被盐析出来。通过色谱分析，转化细胞的S抗原活性存在于2个区域，胚胎细胞的S抗原活性存在于1个区域。其中一个区域在两种细胞提取物中是相同的。
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