Liu Zhongrui, Li Yangyang, Sun Liwen, Yang Hong, Zheng Xin, Wang Libo
Department of Medicinal Chemistry and Natural Medicine Chemistry, College of Pharmacy, Harbin Medical University, Harbin, P. R. China.
Biomed Chromatogr. 2019 Mar;33(3):e4419. doi: 10.1002/bmc.4419. Epub 2018 Nov 28.
Bisphenol A (BPA) is a common additive in food packaging materials, and many methods have been discovered to limit BPA for the sake of food safety. This study aims to find a more sensitive quantification method for BPA. It was determined that a diazo derivative of BPA could be obtained when reacted with a stable and inexpensive reagent, p-methylaniline, exhibiting an increased response in the ultraviolet spectrum than that of nonreacted BPA. Based on this discovery, an accurate method of BPA quantitation was established using a precolumn derivatization HPLC method. The results of this study showed the low limit of detection and the limit of quantitation for BPA were 3.6 and 10.9 pg/mL on-column, respectively. This method is proven to have much higher sensitivity and a lower limit of detection than direct HPLC determination methods. This method is robust, easily handled, sensitive and cost-effective for the quantitation of BPA. In addition, the broad application of this method to determine BPA content in plastic bottled drinking water, distilled water, tap water, milk, Sprite and grape juice was demonstrated in this paper.
双酚A（BPA）是食品包装材料中常见的添加剂，为保障食品安全，人们已发现多种限制双酚A的方法。本研究旨在找到一种更灵敏的双酚A定量方法。研究发现，双酚A与一种稳定且廉价的试剂对甲基苯胺反应时，可得到一种重氮衍生物，该衍生物在紫外光谱中的响应比未反应的双酚A有所增强。基于这一发现，采用柱前衍生化高效液相色谱法建立了一种准确的双酚A定量方法。本研究结果表明，双酚A的柱上检测限和定量限分别为3.6和10.9 pg/mL。事实证明，该方法比直接高效液相色谱测定法具有更高的灵敏度和更低的检测限。该方法稳健、易于操作、灵敏且成本效益高，可用于双酚A的定量分析。此外，本文还展示了该方法在测定塑料瓶装饮用水、蒸馏水、自来水、牛奶、雪碧和葡萄汁中双酚A含量方面的广泛应用。
