Grund A D, Hutchinson C R
J Bacteriol. 1987 Jul;169(7):3013-22. doi: 10.1128/jb.169.7.3013-3022.1987.
Five bacteriophages infecting only Saccharopolyspora erythraea (formerly Streptomyces erythreus) among 43 Streptomyces spp. tested were classified into two groups by phage-host relationships, restriction enzyme mapping, cohesive-end determinations, and Southern hybridizations. phi SE6, the most frequently isolated phage, produced clear plaques on all hosts tested, while phi SE45, phi SE57, phi SE60, and phi SE69 produced turbid plaques. phi SE6 DNA was linear, had a molecular weight of (27.6 +/- 1) X 10(6) and, like the DNAs of phi SE45, phi SE57, and phi SE69, lacked cohesive ends. The characteristic patterns of of ClaI and HindIII restriction digests of phi SE6 DNA and the results of Southern hybridizations with three different ClaI fragments of phi SE6 DNA as probes indicated that phi SE6 DNA was partially circularly permuted and terminally redundant, suggesting that it was packaged by a headful packaging mechanism. Southern hybridization data also showed that phi SE45, phi SE57, and phi SE69 were closely related to phi SE6. phi SE60 DNA, in contrast, had cohesive ends, and restriction mapping plus Southern hybridization data showed that phi SE60 was unrelated to the other four phages.
在测试的43种链霉菌属菌株中，有5种仅感染糖多孢红霉菌（以前称为红色链霉菌）的噬菌体，根据噬菌体-宿主关系、限制性内切酶图谱分析、粘性末端测定和Southern杂交被分为两组。最常分离到的噬菌体phi SE6在所有测试宿主上产生清晰噬菌斑，而phi SE45、phi SE57、phi SE60和phi SE69产生浑浊噬菌斑。phi SE6 DNA是线性的，分子量为(27.6±1)×10⁶，并且与phi SE45、phi SE57和phi SE69的DNA一样，缺乏粘性末端。phi SE6 DNA的ClaI和HindIII限制性酶切特征图谱以及以phi SE6 DNA的三个不同ClaI片段为探针的Southern杂交结果表明，phi SE6 DNA是部分环状排列且末端冗余的，这表明它是通过头部充满式包装机制进行包装的。Southern杂交数据还表明，phi SE45、phi SE57和phi SE69与phi SE6密切相关。相比之下，phi SE60 DNA具有粘性末端，限制性图谱分析和Southern杂交数据表明phi SE60与其他四种噬菌体无关。
