Dept. of Biological and Material Sciences in Dentistry, School of Dentistry, Faculty of Health, Witten/Herdecke University, Witten, Germany.
Dept. of Biological and Material Sciences in Dentistry, School of Dentistry, Faculty of Health, Witten/Herdecke University, Witten, Germany.
Arch Oral Biol. 2019 Jan;97:91-96. doi: 10.1016/j.archoralbio.2018.10.013. Epub 2018 Oct 17.
The aim of this study was to investigate the effects of sodium fluoride (NaF) and amine fluoride (AmF) on bacterial viability in the oral cavity.
Healthy subjects brushed their teeth with either fluoride free toothpaste, NaF- or AmF-containing toothpaste. Biofilm smears from different locations were collected before and immediately and 30 and 120 min after tooth brushing. The smears were stained with live/dead bacterial staining, and the number of the respective bacteria was counted. The data were statistically analyzed by comparing the numbers of bacteria before and after the application of no fluoride, NaF and AmF.
The highest numbers of bacteria were found in the tongue biofilm, followed by the palatal and cheek biofilm. The lowest numbers were found in the mouth floor biofilm. After the application of AmF, no changes in the numbers of bacteria were found in the biofilms, except for the cheek, where they were reduced. After the application of NaF, the number of bacteria decreased significantly in all biofilms. After 120 min, bacterial regrowth was complete.
AmF has only little effect on the bacterial viability of oral biofilms. NaF application reduces the number of living bacteria in the oral biofilms. This effect lasts not longer than 120 min.
本研究旨在探讨氟化钠（NaF）和胺氟化物（AmF）对口腔内细菌活力的影响。
健康受试者使用无氟、含 NaF 或含 AmF 的牙膏刷牙。在刷牙前、刷牙后即刻、30 分钟和 120 分钟，分别从不同部位采集牙菌斑涂片。将涂片用活菌/死菌染色，计数相应细菌数量。通过比较无氟、NaF 和 AmF 应用前后细菌数量，对数据进行统计学分析。
舌部牙菌斑中的细菌数量最多，其次是腭部和颊部牙菌斑，口底牙菌斑中的细菌数量最少。应用 AmF 后，除颊部外，牙菌斑中的细菌数量没有变化，颊部的细菌数量减少。应用 NaF 后，所有牙菌斑中的细菌数量均显著减少。120 分钟后，细菌完全恢复生长。
AmF 对口腔牙菌斑的细菌活力影响较小。NaF 应用可减少口腔牙菌斑中的活菌数量。这种效果持续时间不超过 120 分钟。
