Kelley Brittni R, Ellis J Christopher, Hyatt Doug, Jacobson Dan, Johnson Jeremiah
Department of Microbiology, University of Tennessee, Knoxville, Tennessee.
Oak Ridge National Laboratory, Oak Ridge, Tennessee.
Curr Protoc Microbiol. 2018 Nov;51(1):e64. doi: 10.1002/cpmc.64. Epub 2018 Oct 25.
As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacter has a significant impact on human health. In the developed world, most campylobacteriosis cases are attributed to the consumption of undercooked, contaminated poultry; however, it has been shown that Campylobacter can be transmitted to humans through contaminated water and other types of food, including beef and milk. As such, high-resolution microbial source-tracking is essential for health department officials to determine the source(s) of Campylobacter outbreaks. For these reasons, this protocol provides the techniques needed for isolation of Campylobacter from agricultural and environmental sources, as well as human clinical specimens. Additionally, we describe a simple method for preparing high-quality genomic DNA that can be used for whole-genome sequencing and downstream bioinformatics analyses of Campylobacter genotypes. © 2018 by John Wiley & Sons, Inc.
作为全球细菌性肠胃炎的主要病因，弯曲杆菌对人类健康有重大影响。在发达国家，大多数弯曲杆菌病病例归因于食用未煮熟的受污染家禽；然而，研究表明弯曲杆菌可通过受污染的水和其他类型的食物（包括牛肉和牛奶）传播给人类。因此，高分辨率微生物源追踪对于卫生部门官员确定弯曲杆菌疫情的源头至关重要。基于这些原因，本方案提供了从农业和环境来源以及人类临床标本中分离弯曲杆菌所需的技术。此外，我们描述了一种制备高质量基因组DNA的简单方法，该DNA可用于弯曲杆菌基因型的全基因组测序和下游生物信息学分析。© 2018约翰威立国际出版公司。
