Pourgheysari Batoul, Karimi Leila, Bagheri Raihaneh, Kheiri Soleiman
1Department of Hematology, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Rahmatiyeh, Shahrekord, Iran.
Indian J Hematol Blood Transfus. 2018 Oct;34(4):653-661. doi: 10.1007/s12288-018-0939-8. Epub 2018 Mar 7.
Several studies have demonstrated T cell alteration and some features of immunosenescence in thalassemia major. Repeated alloimmunization converts naïve T-cells to memory cells and iron overload causes oxidative stress accelerating immune aging. To determine whether the alteration of T-cell cytokine is matched with early immune aging, the quantity of cytokine expressing T cells and their correlation to some immune aging markers were investigated. The proportion of IL2- and IFNγ expressing CD4+ and CD8+ T-cells was measured in 27 hepatitis B, C and HIV negative B-thalassemia patients and a control group aged 10-30 years, following stimulation for 6 h with streptococcus enterotoxin B and intracellular cytokine staining. This proportion then were analyzed versus the percentage of the T-cells expressing each phenotyping marker, CD27, CD28, CD57 and CCR7. CD4+ and CD8+ positive T cells expressing IL-2 were significantly lower in β-thalassemia major compared to matched controls, but not T cells expressing IFNγ. No significant difference was observed between splenectomized and non-splenectomized patients in cytokine expressing T cells. A negative correlation was noted between the percentage of T cells expressing IFNγ and T-cells expressing CD-27, but not other markers. Lower T cells expressing IL-2 may reveal the decline of naïve and central memory T cells and is likely to be a feature of early immune aging. Decreased antigenic stimulation and iron overload may help to prevent this phenomenon.
多项研究已证实重型地中海贫血存在T细胞改变及免疫衰老的一些特征。反复的同种免疫会使初始T细胞转变为记忆细胞，而铁过载会导致氧化应激，加速免疫衰老。为了确定T细胞细胞因子的改变是否与早期免疫衰老相匹配，研究了表达细胞因子的T细胞数量及其与一些免疫衰老标志物的相关性。在用链球菌肠毒素B刺激6小时并进行细胞内细胞因子染色后，测量了27例乙肝、丙肝和HIV阴性的β地中海贫血患者以及一个年龄在10至30岁的对照组中表达IL2和IFNγ的CD4+和CD8+ T细胞的比例。然后将该比例与表达每种表型标志物CD27、CD28、CD57和CCR7的T细胞百分比进行分析。与匹配的对照组相比，重型β地中海贫血中表达IL-2的CD4+和CD8+阳性T细胞显著降低，但表达IFNγ的T细胞没有降低。在表达细胞因子的T细胞方面，脾切除患者和未脾切除患者之间未观察到显著差异。观察到表达IFNγ的T细胞百分比与表达CD-27的T细胞之间呈负相关，但与其他标志物无关。表达IL-2的T细胞减少可能揭示了初始和中央记忆T细胞的减少，并且很可能是早期免疫衰老的一个特征。抗原刺激减少和铁过载可能有助于预防这种现象。
