Park Jungkum, Lee Gyu Min, Kim Donghyuk, Park Duck Hwan, Oh Chang-Sik
Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea.
Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea.
Plant Pathol J. 2018 Oct;34(5):445-450. doi: 10.5423/PPJ.NT.06.2018.0100. Epub 2018 Oct 1.
Bacteriophages, bacteria-infecting viruses, have been recently reconsidered as a biological control tool for preventing bacterial pathogens. and cause fire blight and black shoot blight disease in apple and pear, respectively. In this study, the bacteriophage phiEaP-8 was isolated from apple orchard soil and could efficiently and specifically kill both and . This bacteriophage belongs to the family. Whole genome analysis revealed that phiEaP-8 carries a 75,929 bp genomic DNA with 78 coding sequences and 5 tRNA genes. Genome comparison showed that phiEaP-8 has only 85% identity to known bacteriophages at the DNA level. PhiEaP-8 retained lytic activity up to 50°C, within a pH range from 5 to 10, and under 365 nm UV light. Based on these characteristics, the bacteriophage phiEaP-8 is novel and carries potential to control both and in apple and pear.
噬菌体是感染细菌的病毒，最近被重新视为一种预防细菌病原体的生物控制工具。[此处原文缺失两种细菌的名称]分别在苹果和梨中引起火疫病和黑枝疫病。在本研究中，噬菌体phiEaP - 8是从苹果园土壤中分离出来的，它能够高效且特异性地杀死[此处原文缺失两种细菌的名称]。这种噬菌体属于[此处原文缺失噬菌体所属家族名称]家族。全基因组分析表明，phiEaP - 8携带一个75,929 bp的基因组DNA，有78个编码序列和5个tRNA基因。基因组比较显示，phiEaP - 8在DNA水平上与已知噬菌体的同一性仅为85%。phiEaP - 8在高达50°C的温度、5至10的pH范围内以及365 nm紫外光下都能保持裂解活性。基于这些特性，噬菌体phiEaP - 8是新型的，具有控制苹果和梨中[此处原文缺失两种细菌的名称]的潜力。
