Jo Su Jin, Kim Sang Guen, Lee Young Min, Giri Sib Sankar, Kang Jeong Woo, Lee Sung Bin, Jung Won Joon, Hwang Mae Hyun, Park Jaehong, Cheng Chi, Roh Eunjung, Park Se Chang
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.
Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Biology (Basel). 2023 Jan 23;12(2):180. doi: 10.3390/biology12020180.
The recent outbreak of blight in pome fruit plants has been a major concern as there are two indistinguishable species, and , which cause blight in South Korea. Although there is a strict management protocol consisting of antibiotic-based prevention, the area and the number of cases of outbreaks have increased. In this study, we isolated four bacteriophages, pEp_SNUABM_03, 04, 11, and 12, that infect both and and evaluated their potential as antimicrobial agents for administration against -originated blight in South Korea. Morphological analysis revealed that all phages had podovirus-like capsids. The phage cocktail showed a broad spectrum of infectivity, infecting 98.91% of and 100% of strains. The antibacterial effect was observed after long-term cocktail treatment against , whereas it was observed for both short- and long-term treatments against . Genomic analysis verified that the phages did not encode harmful genes such as antibiotic resistance or virulence genes. All phages were stable under general orchard conditions. Collectively, we provided basic data on the potential of phages as biocontrol agents that target both and
最近在仁果类果树中爆发的疫病令人十分担忧，因为在韩国有两种难以区分的物种，即[物种A]和[物种B]，它们会引发疫病。尽管存在由基于抗生素的预防措施组成的严格管理方案，但疫病爆发的面积和病例数仍在增加。在本研究中，我们分离出了四种噬菌体，即pEp_SNUABM_03、04、11和12，它们可同时感染[物种A]和[物种B]，并评估了它们作为抗微生物剂用于防治韩国[物种A]引发的疫病的潜力。形态学分析表明，所有噬菌体都具有类短尾病毒的衣壳。噬菌体混合物表现出广泛的感染性，可感染98.91%的[物种A]菌株和100%的[物种B]菌株。对[物种A]进行长期混合物处理后观察到了抗菌效果，而对[物种B]进行短期和长期处理均观察到了抗菌效果。基因组分析证实，这些噬菌体不编码抗生素抗性或毒力基因等有害基因。所有噬菌体在一般果园条件下都很稳定。总体而言，我们提供了关于噬菌体作为针对[物种A]和[物种B]的生物防治剂潜力的基础数据。
