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在韩国分离出九种对梨火疫病菌有效的噬菌体。

Isolation of Nine Bacteriophages Shown Effective against Erwinia amylovora in Korea.

作者信息

Park Jungkum, Kim Byeori, Song Sujin, Lee Yong Whan, Roh Eunjung

机构信息

Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.

出版信息

Plant Pathol J. 2022 Jun;38(3):248-253. doi: 10.5423/PPJ.NT.11.2021.0172. Epub 2022 Jun 1.

DOI:10.5423/PPJ.NT.11.2021.0172
PMID:35678058
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9343912/
Abstract

Erwinia amylovora is a devastating bacterial plant pathogen that infects Rosaceae including apple and pear and causes fire blight. Bacteriophages have been considered as a biological control agent for preventing bacterial infections of plants. In this study, nine bacteriophages (ΦFifi011, ΦFifi044, ΦFifi051, ΦFifi067, ΦFifi106, ΦFifi287, ΦFifi318, ΦFifi450, and ΦFifi451) were isolated from soil and water samples in seven orchards with fire blight in Korea. The genetic diversity of bacteriophage isolates was confirmed through restriction fragment length polymorphism pattern analysis. Host range of the nine phages was tested against 45 E. amylovora strains and 14 E. pyrifoliae strains and nine other bacterial strains. Among the nine phages, ΦFifi044 and ΦFifi451 infected and lysed E. amylovora only. And the remaining seven phages infected both E. amylovora and E. pyrifoliae. The results suggest that the isolated phages were different from each other and effective to control E. amylovora, providing a basis to develop biological agents and utilizing phage cocktails.

摘要

梨火疫病菌是一种具有毁灭性的细菌性植物病原体，可感染包括苹果和梨在内的蔷薇科植物并引发火疫病。噬菌体已被视为预防植物细菌感染的生物防治剂。在本研究中，从韩国七个发生火疫病的果园的土壤和水样中分离出九种噬菌体（ΦFifi011、ΦFifi044、ΦFifi051、ΦFifi067、ΦFifi106、ΦFifi287、ΦFifi318、ΦFifi450和ΦFifi451）。通过限制性片段长度多态性模式分析证实了噬菌体分离株的遗传多样性。针对45株梨火疫病菌菌株、14株梨叶欧文氏菌菌株和其他九种细菌菌株测试了这九种噬菌体的宿主范围。在这九种噬菌体中，ΦFifi044和ΦFifi451仅感染并裂解梨火疫病菌。其余七种噬菌体则同时感染梨火疫病菌和梨叶欧文氏菌。结果表明，分离出的噬菌体彼此不同，对控制梨火疫病菌有效，为开发生物制剂和利用噬菌体混合物提供了依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08ad/9343912/1a2cd817587f/ppj-nt-11-2021-0172f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08ad/9343912/1a2cd817587f/ppj-nt-11-2021-0172f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08ad/9343912/1a2cd817587f/ppj-nt-11-2021-0172f3.jpg

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