单纯疱疹病毒1型的65,000道尔顿DNA结合及病毒体反式诱导蛋白
The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.
作者信息
Marsden H S, Campbell M E, Haarr L, Frame M C, Parris D S, Murphy M, Hope R G, Muller M T, Preston C M
出版信息
J Virol. 1987 Aug;61(8):2428-37. doi: 10.1128/JVI.61.8.2428-2437.1987.
Abstract
The possible identity of the herpes simplex virus type 1 (HSV-1) 65K (65,000-Mr) virion protein which stimulates transcription from immediate-early genes with the HSV-1 65K DNA-binding protein was investigated. The two proteins were found to be distinct by the three separate criteria of immunological reactivity, tryptic peptide fingerprinting, and mobility in two-dimensional gels. Using HSV-1/HSV-2 intertypic recombinants and a serotype-specific antiserum, we located the gene encoding the 65K DNA-binding protein between coordinates 0.574 and 0.682 on the HSV-1 genome. The protein is posttranslationally modified by phosphorylation. In crude extracts of HSV-1-infected cells the 65K trans-inducing protein did not detectably bind to double-stranded calf thymus DNA under the conditions of our assay.
摘要
对单纯疱疹病毒1型（HSV-1）刺激即刻早期基因转录的65K（65,000道尔顿）病毒体蛋白与HSV-1 65K DNA结合蛋白的可能同一性进行了研究。通过免疫反应性、胰蛋白酶肽指纹图谱和二维凝胶中的迁移率这三个独立标准发现这两种蛋白是不同的。利用HSV-1/HSV-2型间重组体和血清型特异性抗血清，我们将编码65K DNA结合蛋白的基因定位在HSV-1基因组坐标0.574和0.682之间。该蛋白通过磷酸化进行翻译后修饰。在我们的检测条件下，在HSV-1感染细胞的粗提物中，65K反式诱导蛋白未检测到与双链小牛胸腺DNA结合。
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