Takimoto T, Sato H, Ogura H, Ishikawa S, Miyazaki T
ORL J Otorhinolaryngol Relat Spec. 1987;49(2):81-6. doi: 10.1159/000275911.
Transfection experiments using Epstein-Barr virus (EBV) DNA were carried out to examine whether three different epithelial cell lines (Ad-AH, D98, and KB) could be transfected and whether biologically active virus could be recovered. We found that the Ad-AH cells derived from the human nasopharynx were more transfectable, as determined by the synthesis of EBV-specific antigens. Concentrates of supernatant fluids obtained from transfected Ad-AH cells were capable of transforming human cord blood lymphocytes (HCBLs). However, no concentrates of supernatant fluids obtained from transfected epithelial cell lines outside the nasopharynx (D98 and KB) were capable of transforming HCBLs.
进行了使用爱泼斯坦-巴尔病毒（EBV）DNA的转染实验，以检查三种不同的上皮细胞系（Ad-AH、D98和KB）是否能够被转染，以及是否能够回收具有生物活性的病毒。我们发现，通过EBV特异性抗原的合成确定，源自人鼻咽的Ad-AH细胞更易于被转染。从转染的Ad-AH细胞获得的上清液浓缩物能够转化人脐血淋巴细胞（HCBL）。然而，从鼻咽外的转染上皮细胞系（D98和KB）获得的上清液浓缩物均不能转化HCBL。
