Sharma J M, Fredericksen T L
Prog Clin Biol Res. 1987;238:283-94.
Spleen cells of chickens infected with IBDV responded poorly to in vitro stimulation with Con A. The mitogenic hyporesponsiveness was due to the presence of suppressor cells that could be removed by pretreatment of IS cells with carbonyl iron or cytodex-3 microcarrier beads. Addition of suppressor cells to normal spleen cells prevented the normal cells from responding to mitogen. Cell-to-cell contact between responder and suppressor cells was not necessary for suppression to occur; the effect was mediated by soluble product(s) released by the suppressor cells. IS cells were also deficient in producing mitogen-induced IL-2 and addition of exogenous IL-2 did not restore mitogenic response of IS.
感染传染性法氏囊病病毒（IBDV）的鸡的脾细胞对刀豆蛋白A（Con A）的体外刺激反应不佳。促有丝分裂低反应性是由于存在抑制细胞，这些抑制细胞可以通过用羰基铁或cytodex - 3微载体珠对免疫抑制（IS）细胞进行预处理来去除。将抑制细胞添加到正常脾细胞中会阻止正常细胞对有丝分裂原产生反应。抑制作用的发生并不需要反应细胞和抑制细胞之间的细胞间接触；这种效应是由抑制细胞释放的可溶性产物介导的。IS细胞在产生有丝分裂原诱导的白细胞介素-2（IL - 2）方面也存在缺陷，并且添加外源性IL - 2并不能恢复IS的促有丝分裂反应。
