狨猴（Callithrix jacchus）前额皮质的性别偏向性基因表达及其潜在的行为相关性。
Sex-biased gene expression in the frontal cortex of common marmosets (Callithrix jacchus) and potential behavioral correlates.
机构信息
Health Sciences Graduate Program, Federal University of Rio Grande do Norte, Natal, Brazil.
Bioinformatics Multidisciplinary Environment, Federal University of Rio Grande do Norte, Natal, Brazil.
出版信息
Brain Behav. 2018 Dec;8(12):e01148. doi: 10.1002/brb3.1148. Epub 2018 Oct 30.
INTRODUCTION
The common marmoset (Callithrix jacchus), a small New World monkey, has been widely used as a biological model in neuroscience to elucidate neural circuits involved in cognition and to understand brain dysfunction in neuropsychiatric disorders. In this regard, the availability of gene expression data derived from next-generation sequencing (NGS) technologies represents an opportunity for a molecular contextualization. Sexual dimorphism account for differences in diseases prevalence and prognosis. Here, we explore sex differences on frontal cortex of gene expression in common marmoset's adults.
METHODS
Gene expression profiles in six different tissues (cerebellum, frontal cortex, liver, heart, and kidney) were analyzed in male and female marmosets. To emphasize the translational value of this species for behavioral studies, we focused on sex-biased gene expression from the frontal cortex of male and female in common marmosets and compared to humans (Homo sapiens).
RESULTS
In this study, we found that frontal cortex genes whose expression is male-biased are conserved between marmosets and humans and enriched with "house-keeping" functions. On the other hand, female-biased genes are more related to neural plasticity functions involved in remodeling of synaptic circuits, stress cascades, and visual behavior. Additionally, we developed and made available an application-the CajaDB-to provide a friendly interface for genomic, expression, and alternative splicing data of marmosets together with a series of functionalities that allow the exploration of these data. CajaDB is available at cajadb.neuro.ufrn.br.
CONCLUSION
The data point to differences in gene expression of male and female common marmosets in all tissues analyzed. In frontal cortex, female-biased expression in synaptic plasticity, stress, and visual processing might be linked to biological and behavioral mechanisms of this sex. Due to the limited sample size, the data here analyzed are for exploratory purposes.
简介
普通卷尾猴（Callithrix jacchus）是一种小型新世界猴，已被广泛用作神经科学中的生物模型，以阐明参与认知的神经回路，并了解神经精神障碍中的大脑功能障碍。在这方面，源自下一代测序（NGS）技术的基因表达数据的可用性代表了分子上下文的机会。性别二态性解释了疾病流行率和预后的差异。在这里，我们探讨了成年普通卷尾猴额叶皮层基因表达的性别差异。
方法
分析了 6 种不同组织（小脑、额叶皮层、肝脏、心脏和肾脏）中雄性和雌性卷尾猴的基因表达谱。为了强调该物种对行为研究的转化价值，我们专注于普通卷尾猴额叶皮层中雄性和雌性的性别偏倚基因表达，并与人类（Homo sapiens）进行了比较。
结果
在这项研究中，我们发现，在卷尾猴和人类之间，表达呈雄性偏倚的额叶皮层基因是保守的，并且富集了“管家”功能。另一方面，雌性偏倚的基因更多地与神经可塑性功能有关，这些功能涉及突触回路、应激级联和视觉行为的重塑。此外，我们开发并提供了一个应用程序-CajaDB-为卷尾猴的基因组、表达和选择性剪接数据提供了一个友好的界面，并提供了一系列功能，允许探索这些数据。CajaDB 可在 cajadb.neuro.ufrn.br 获得。
结论
这些数据表明，在所分析的所有组织中，雄性和雌性普通卷尾猴的基因表达存在差异。在额叶皮层中，与突触可塑性、应激和视觉处理相关的雌性偏倚表达可能与该性别相关的生物学和行为机制有关。由于样本量有限，这里分析的数据仅用于探索目的。
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