Powell H C, Mizisin A P, Wiley C A, Morey M K, Hughes R A
Acta Neuropathol. 1987;73(1):12-8. doi: 10.1007/BF00695496.
Experimental neuropathy, characterized by endoneurial edema and demyelination, was induced by inoculating rabbits with a combination of Freund's complete adjuvant (FCA), gangliosides, lecithin and cholesterol. A less severe demyelinating neuropathy could be induced by treatment with FCA alone but no significant change could be elicited by injection of swine influenza vaccine (SFV) alone. When FCA was combined with gangliosides, lecithins, cholesterol and SFV, neuropathy occurred, but the changes were less severe than if these agents were used without SFV. Sera were tested for myelin basic protein (MBP) and galactocerebroside (GC) antibodies in each experimental group. Neither SFV alone nor SFV combined with Freund's complete adjuvant, gangliosides, cholesterol and lecithin evoked significant antibody titers to MBP or GC. However, rabbits inoculated with FCA, gangliosides, lecithin and cholesterol had rising titers of antibody to both MBP and GC over the 3-month experimental period. One rabbit inoculated with FCA alone had significant antibody to MBP. The findings suggest that Freund's complete adjuvant alone can induce demyelination in the peripheral nerves of rabbits and that SFV may modulate the immune response acting either as an adjuvant or suppressant in the experimental demyelinating disease.
通过给兔子接种弗氏完全佐剂（FCA）、神经节苷脂、卵磷脂和胆固醇的组合来诱导实验性神经病变，其特征为神经内膜水肿和脱髓鞘。单独用FCA治疗可诱导出较轻的脱髓鞘性神经病变，但单独注射猪流感疫苗（SFV）则不会引起明显变化。当FCA与神经节苷脂、卵磷脂、胆固醇和SFV联合使用时，会发生神经病变，但变化程度比不使用SFV时使用这些药物要轻。对每个实验组的血清进行髓鞘碱性蛋白（MBP）和半乳糖脑苷脂（GC）抗体检测。单独的SFV或与弗氏完全佐剂、神经节苷脂、胆固醇和卵磷脂联合使用的SFV均未引起对MBP或GC的显著抗体滴度。然而，在3个月的实验期内，接种FCA、神经节苷脂、卵磷脂和胆固醇的兔子对MBP和GC的抗体滴度均有所上升。一只单独接种FCA的兔子对MBP有显著抗体。这些发现表明，单独的弗氏完全佐剂可诱导兔子外周神经脱髓鞘，并且SFV可能在实验性脱髓鞘疾病中作为佐剂或抑制剂调节免疫反应。
