Bergmeyer H U
Z Klin Chem Klin Biochem. 1975 Nov;13(11):507-8.
Extensive re-investigations with regard to the molar extinction coefficients of NADH and NADPH proved that in future, calculations in routine work can be performed with the following much more accurate epsilon-values: 6.15 x 10(3) 1 x mol-1 x cm-1 at Hg 334 nm (NADH and NADPH), 6.3 X 10(3) 1 X mol-1 x cm-1 at 340 nm (NADH and NADPH), 3.4 X 10(3) 1 X mol-1 X Cm-1 (NADH) and 3.5 x 10(3) 1 x mol-1 x cm-1 (NADPH) at Hg 365 nm, respectively. The safest measurement is performed at Hg 334 nm, because here epsilon is identical for both coenzymes and deviations of the epsilon-value caused by temperature, pH and ionic strength are less than 0.5%.
关于NADH和NADPH摩尔消光系数的广泛重新研究证明，今后在常规工作中进行计算时，可以使用以下更精确的ε值：在汞灯334nm处为6.15×10³ 1×mol⁻¹×cm⁻¹（NADH和NADPH），在340nm处为6.3×10³ 1×mol⁻¹×cm⁻¹（NADH和NADPH），在汞灯365nm处分别为3.4×10³ 1×mol⁻¹×cm⁻¹（NADH）和3.5×10³ 1×mol⁻¹×cm⁻¹（NADPH）。最安全的测量是在汞灯334nm处进行，因为此处两种辅酶的ε相同，且温度、pH和离子强度引起的ε值偏差小于0.5%。
