CEB, Centre of Biological Engineering, LIBRO-Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, 4710-057 Braga, Portugal.
Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, Devon, UK.
Biomolecules. 2018 Oct 30;8(4):130. doi: 10.3390/biom8040130.
biofilms are recognized to have high resistance to antifungals. In order to understand the effect of mannans in the resistance profile of mature biofilms, Δ was evaluated. Biofilm cell walls were analysed by confocal laser scanning microscopy (CLSM) and their susceptibility was assessed for fluconazole, amphotericin B, caspofungin, and micafungin. Crystal violet and Alcian Blue methods were performed to quantify the biomass and the mannans concentration in the biofilm cells and matrices, respectively. The concentration of β-1,3 glucans was also measured. No visible differences were detected among cell walls of the strains, but the mutant had a high biomass reduction, after a drug stress. When compared with the reference strain, it was detected a decrease in the susceptibility of the biofilm cells and an increase of β-1,3 glucans in the Δ. The deletion of the gene in induces biofilm matrix and cell wall variabilities that increase the resistance to the antifungal drug treatments. The rise of β-1,3 glucans appears to have a role in this effect.
生物膜被认为对抗真菌药物具有高度抗性。为了了解甘露聚糖在成熟生物膜耐药谱中的作用，评估了Δ。通过共聚焦激光扫描显微镜（CLSM）分析生物膜细胞壁，并评估其对氟康唑、两性霉素 B、卡泊芬净和米卡芬净的敏感性。分别采用结晶紫和阿尔辛蓝法定量生物膜细胞和基质中的生物量和甘露聚糖浓度。还测量了β-1,3 葡聚糖的浓度。在菌株细胞壁之间未检测到明显差异，但在药物应激后，突变体的生物量减少幅度较大。与参考菌株相比，发现生物膜细胞的敏感性降低，Δ中的β-1,3 葡聚糖增加。在 中缺失 基因会导致生物膜基质和细胞壁发生变化，从而增加对抗真菌药物治疗的耐药性。β-1,3 葡聚糖的增加似乎在这种作用中起作用。
