Alves Anelise Maria Costa Vasconcelos, Lopes Beatriz Oliveira, Leite Ana Caroline Rocha de Melo, Cruz Gabriela Silva, Brito Érika Helena Salles de, Lima Laritza Ferreira de, Černáková Lucia, Azevedo Nuno Filipe, Rodrigues Célia Fortuna
Institute of Health Sciences, University of International Integration of Af-ro-Brazilian Lusophony, Av. da Abolição, 3-Centro, Redenção 62790-000, Ceará, Brazil.
LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal.
Antibiotics (Basel). 2023 Apr 22;12(5):797. doi: 10.3390/antibiotics12050797.
and non- species (NCACs) are known to colonize and invade various tissues, including the oral mucosa. In this work, we aimed to characterize mature biofilms of several spp. clinical isolates ( = 33) obtained from the oral mucosa of children, adults, and elders of Eastern Europe and South America.
Each strain was evaluated for its capacity to form biofilms in terms of total biomass using the crystal violet assay and for matrix components production (proteins and carbohydrates) using the BCA and phenol-sulfuric tests, respectively. The effect of different antifungals on biofilm formation was studied.
in the children's group, a predominance of (81%) was observed, while, among adults, the main species was (59%). Most strains showed a reduced response to antimicrobial drugs when in biofilm form ( < 0.01). Moreover, it was observed that strains isolated from children produced more matrix, with higher levels of protein and polysaccharides.
children were more likely to be infected by NCACs than adults. More importantly, these NCACs were able to form biofilms richer in matrix components. This finding is of clinical importance, particularly in pediatric care, since stronger biofilms are highly associated with antimicrobial resistance, recurrent infections, and higher therapeutic failure.
非念珠菌属念珠菌（NCACs）已知可定殖并侵入包括口腔黏膜在内的各种组织。在本研究中，我们旨在对从东欧和南美洲儿童、成人及老年人口腔黏膜分离得到的几种念珠菌临床分离株（n = 33）的成熟生物膜进行表征。
使用结晶紫测定法评估每个菌株形成生物膜的总生物量能力，并分别使用BCA和苯酚 - 硫酸法检测基质成分（蛋白质和碳水化合物）的产生。研究了不同抗真菌剂对生物膜形成的影响。
在儿童组中，观察到热带念珠菌占主导地位（81%），而在成人中，主要菌种是白色念珠菌（59%）。大多数菌株在形成生物膜时对抗菌药物的反应降低（P < 0.01）。此外，观察到从儿童分离的菌株产生更多的基质，蛋白质和多糖水平更高。
儿童比成人更容易感染非念珠菌属念珠菌。更重要的是，这些非念珠菌属念珠菌能够形成富含基质成分的生物膜。这一发现具有临床重要性，特别是在儿科护理中，因为更强的生物膜与抗菌药物耐药性、复发性感染和更高的治疗失败率高度相关。
