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人体细胞外液的抗氧化作用。人血清及其蛋白质成分对次氯酸和过氧化氢使α1-抗蛋白酶失活的影响。

The antioxidant action of human extracellular fluids. Effect of human serum and its protein components on the inactivation of alpha 1-antiproteinase by hypochlorous acid and by hydrogen peroxide.

作者信息

Wasil M, Halliwell B, Hutchison D C, Baum H

出版信息

Biochem J. 1987 Apr 1;243(1):219-23. doi: 10.1042/bj2430219.

DOI:10.1042/bj2430219
PMID:3038080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147835/
Abstract

The elastase-inhibitory capacity of purified human alpha 1-antiproteinase is inactivated by low concentrations of the myeloperoxidase-derived oxidant hypochlorous acid, but much higher concentrations are required to inhibit the elastase-inhibitory capacity of serum samples. The protective effect of serum appears to be largely due to albumin. High concentrations of H2O2 also inactivate the elastase-inhibitory capacity of alpha 1-antiproteinase, by a mechanism not involving formation of hydroxyl radicals. Serum offers protection against H2O2 inactivation of alpha 1-antiproteinase. The relevance of these results to the tissue damage produced by activated phagocytes is discussed.

摘要

纯化的人α1抗蛋白酶的弹性蛋白酶抑制能力可被低浓度的髓过氧化物酶衍生的氧化剂次氯酸灭活，但需要高得多的浓度才能抑制血清样本的弹性蛋白酶抑制能力。血清的保护作用似乎主要归因于白蛋白。高浓度的H2O2也会通过不涉及羟基自由基形成的机制使α1抗蛋白酶的弹性蛋白酶抑制能力失活。血清可提供针对H2O2使α1抗蛋白酶失活的保护作用。讨论了这些结果与活化吞噬细胞产生的组织损伤的相关性。

相似文献

1
The antioxidant action of human extracellular fluids. Effect of human serum and its protein components on the inactivation of alpha 1-antiproteinase by hypochlorous acid and by hydrogen peroxide.人体细胞外液的抗氧化作用。人血清及其蛋白质成分对次氯酸和过氧化氢使α1-抗蛋白酶失活的影响。
Biochem J. 1987 Apr 1;243(1):219-23. doi: 10.1042/bj2430219.
2
The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid.N-乙酰半胱氨酸的抗氧化作用：其与过氧化氢、羟基自由基、超氧化物和次氯酸的反应。
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3
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J Immunol. 1986 Jan;136(2):636-41.

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本文引用的文献

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Assessment of chlorination by human neutrophils.人中性粒细胞对氯化作用的评估。
Nature. 1983 Feb 24;301(5902):715-6. doi: 10.1038/301715a0.
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Free radical pathology: inactivation of human alpha-1-proteinase inhibitor by products from the reaction of nitrogen dioxide with hydrogen peroxide and the etiology of emphysema.自由基病理学：二氧化氮与过氧化氢反应产物对人α-1-蛋白酶抑制剂的失活作用及肺气肿的病因学
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Proteases and lung injury. A state-of-the-art minireview.蛋白酶与肺损伤。一篇前沿综述。
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Oxidative mechanisms utilized by human neutrophils to destroy Escherichia coli.人类中性粒细胞用于破坏大肠杆菌的氧化机制。
Blood. 1984 Jun;63(6):1361-8.
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Ceruloplasmin: plasma inhibitor of the oxidative inactivation of alpha 1-protease inhibitor.铜蓝蛋白：α1-蛋白酶抑制剂氧化失活的血浆抑制剂。
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Chlorination of taurine by human neutrophils. Evidence for hypochlorous acid generation.人中性粒细胞对牛磺酸的氯化作用。次氯酸生成的证据。
J Clin Invest. 1982 Sep;70(3):598-607. doi: 10.1172/jci110652.
9
Oxidative inactivation of Escherichia coli by hypochlorous acid. Rates and differentiation of respiratory from other reaction sites.次氯酸对大肠杆菌的氧化失活作用。呼吸作用与其他反应位点的速率及差异
FEBS Lett. 1982 Jul 19;144(1):157-61. doi: 10.1016/0014-5793(82)80591-7.
10
Production of superoxide, hydrogen peroxide and hydroxyl radicals by phagocytic cells: a cause of chronic inflammatory disease?吞噬细胞产生超氧化物、过氧化氢和羟基自由基：慢性炎症性疾病的一个病因？
Cell Biol Int Rep. 1982 Jun;6(6):529-42. doi: 10.1016/0309-1651(82)90175-8.