硫脲、二甲基硫脲和二甲基亚砜作为羟基自由基清除剂的特异性。它们对α1-抗蛋白酶免受次氯酸灭活的保护作用。
The specificity of thiourea, dimethylthiourea and dimethyl sulphoxide as scavengers of hydroxyl radicals. Their protection of alpha 1-antiproteinase against inactivation by hypochlorous acid.
作者信息
Wasil M, Halliwell B, Grootveld M, Moorhouse C P, Hutchison D C, Baum H
机构信息
Department of Thoracic Medicine, Faculty of Clinical Medicine, King's College (KQC), Denmark Hill, London, U.K.
出版信息
Biochem J. 1987 May 1;243(3):867-70. doi: 10.1042/bj2430867.
Abstract
Thiourea and dimethylthiourea are powerful scavengers of hydroxyl radicals (.OH), and dimethylthiourea has been used to test the involvement of .OH in several animal models of human disease. It is shown that both thiourea and dimethylthiourea are scavengers of HOCl, a powerful oxidant produced by neutrophil myeloperoxidase. Hence the ability of dimethylthiourea to protect against neutrophil-mediated tissue damage cannot be used as evidence for a role of .OH in causing such damage. Dimethyl sulphoxide also reacts with HOCl, but at a rate that is probably too low to be biologically significant at dimethyl sulphoxide concentrations up to 10 mM. Neither mannitol nor desferrioxamine, at the concentrations normally used in radical-generating systems, appears to react with HOCl.
摘要
硫脲和二甲基硫脲是羟基自由基（·OH）的强效清除剂，二甲基硫脲已被用于在多种人类疾病动物模型中检测·OH的参与情况。结果表明，硫脲和二甲基硫脲都是次氯酸（HOCl）的清除剂，次氯酸是中性粒细胞髓过氧化物酶产生的一种强氧化剂。因此，二甲基硫脲预防中性粒细胞介导的组织损伤的能力不能用作·OH在引起此类损伤中起作用的证据。二甲基亚砜也与HOCl反应，但在二甲基亚砜浓度高达10 mM时，其反应速率可能过低，不具有生物学意义。在自由基生成系统中通常使用的浓度下，甘露醇和去铁胺似乎都不与HOCl反应。
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本文引用的文献
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Am Rev Respir Dis. 1982 Nov;126(5):802-6. doi: 10.1164/arrd.1982.126.5.802.
2
In vivo damage of rat lungs by oxygen metabolites.氧代谢产物对大鼠肺的体内损伤。
J Clin Invest. 1981 Apr;67(4):983-93. doi: 10.1172/jci110149.
3
Evidence for role of hydroxyl radical in complement and neutrophil-dependent tissue injury.羟自由基在补体和中性粒细胞依赖性组织损伤中的作用证据。
J Clin Invest. 1983 Sep;72(3):789-801. doi: 10.1172/JCI111050.
4
Prevention of thiourea-induced pulmonary edema by hydroxyl-radical scavengers.羟基自由基清除剂对硫脲诱导的肺水肿的预防作用
J Appl Physiol Respir Environ Exerc Physiol. 1983 Nov;55(5):1456-9. doi: 10.1152/jappl.1983.55.5.1456.
5
Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions.氧衍生自由基和代谢产物在白细胞依赖性炎症反应中的作用。
Am J Pathol. 1982 Jun;107(3):395-418.
6
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7
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8
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10
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