Foote C S, Goyne T E, Lehrer R I
Nature. 1983 Feb 24;301(5902):715-6. doi: 10.1038/301715a0.
On phagocytosing a microorganism, the neutrophil (polymorphonuclear leukocyte, PMN) consumes oxygen at a sharply elevated rate1. The oxygen is used to kill the microorganism, presumably being used to produce a potent oxidizing agent or agents. Candidates for these bactericidal agents are singlet oxygen, hydroxyl radical and chlorinating agents (that is, species containing 'active' Cl in a formal +1 oxidation state: HOCl, Cl2, N-chloroamides, and so on)1-5. We now report a semiquantitative assay for PMN-generated active chlorine based on its trapping with 1,3,5-trimethoxybenzene (TMB). Using this assay, we have found that at least 28% of the oxygen consumed by stimulated normal human PMNs is converted to active chlorinating agents.
在吞噬微生物时，中性粒细胞（多形核白细胞，PMN）会以急剧升高的速率消耗氧气。这些氧气用于杀死微生物，推测是用于产生一种或多种强效氧化剂。这些杀菌剂的候选物是单线态氧、羟基自由基和氯化剂（即含有处于形式 +1 氧化态的“活性”Cl 的物种：次氯酸、氯气、N-氯代酰胺等）。我们现在报告一种基于用 1,3,5-三甲氧基苯（TMB）捕获来对PMN 产生的活性氯进行半定量测定的方法。使用这种测定方法，我们发现受刺激的正常人PMN 消耗的氧气中至少 28% 会转化为活性氯化剂。
