Lidor C, Meyer M S, Wasserman R H, Edelstein S
Biochem J. 1987 Apr 1;243(1):75-8. doi: 10.1042/bj2430075.
Decreased intestinal absorption of Ca2+ occurs in response to treatment with disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP). The effect is due to decreased 1-hydroxylation of calcidiol (25-hydroxycholecalciferol) in the kidney. In an attempt to establish whether impairment of vitamin D metabolism at steps beyond kidney hydroxylation occurs due to treatment with EHDP, chicks were depleted of vitamin D and were treated with calcitriol (1,25-dihydroxycholecalciferol) as their sole source of the vitamin. The chicks were then divided into two groups, one being treated with EHDP while the second group served as control. Intestinal absorption of Ca2+ in the EHDP-treated group was found to be impaired, along with decreases in concentrations of calbindin D28K (the 28,000-Mr vitamin D-dependent Ca2+-binding protein). When the chicks were dosed with [3H]calcitriol, significantly lower concentrations of the sterol were detected in the duodena of EHDP-treated birds. Measurement of levels of receptors for calcitriol in duodena showed no difference between groups, but levels of calcitriol in sera were considerably lower in the EHDP-treated group along with the elevated biliary and urinary excretion of glucuronidated conjugates. It is therefore concluded that treatment with EHDP results in increased catabolism of calcitriol in addition to the known suppression of the renal production of the hormone.
用乙烷-1-羟基-1,1-二膦酸二钠（EHDP）治疗会导致肠道对Ca2+的吸收减少。这种作用是由于肾脏中骨化二醇（25-羟基胆钙化醇）的1-羟化作用降低所致。为了确定用EHDP治疗是否会在肾脏羟化作用之后的步骤导致维生素D代谢受损，将雏鸡的维生素D耗尽，并用骨化三醇（1,25-二羟基胆钙化醇）作为其唯一的维生素来源进行治疗。然后将雏鸡分为两组，一组用EHDP治疗，另一组作为对照。发现EHDP治疗组的肠道对Ca2+的吸收受损，同时钙结合蛋白D28K（分子量为28,000的维生素D依赖性Ca2+结合蛋白）的浓度降低。当给雏鸡注射[3H]骨化三醇时，在EHDP治疗的雏鸡十二指肠中检测到的固醇浓度明显较低。对十二指肠中骨化三醇受体水平的测量显示两组之间没有差异，但EHDP治疗组血清中骨化三醇的水平明显较低，同时葡糖醛酸化结合物的胆汁和尿液排泄增加。因此得出结论，用EHDP治疗除了已知会抑制该激素的肾脏产生外，还会导致骨化三醇的分解代谢增加。
