恶性疟原虫含潜在编码富含组氨酸蛋白重复区域的DNA片段的克隆与测序：两个重叠阅读框的鉴定

Cloning and sequencing of Plasmodium falciparum DNA fragments containing repetitive regions potentially coding for histidine-rich proteins: identification of two overlapping reading frames.

作者信息

Lenstra R, d'Auriol L, Andrieu B, Le Bras J, Galibert F

出版信息

Biochem Biophys Res Commun. 1987 Jul 15;146(1):368-77. doi: 10.1016/0006-291x(87)90734-0.

Abstract

DNA sequences, potentially coding for histidine-rich proteins, were isolated from a P. falciparum genomic library using an oligonucleotide probe consisting of histidine codon repeats. Sequencing revealed that the different DNA fragments contain long repetitive regions very homologous to the probe. One clone was fully sequenced and contains two open reading frames that overlap in the repetitive region but are located on opposite strands. Analysis suggests that both are coding. One frame could code for a small histidine-rich protein, the other for a protein containing many aspartic acid residues. Southern blotting revealed that these sequences are conserved in all three P. falciparum strains studied.

摘要

使用由组氨酸密码子重复序列组成的寡核苷酸探针，从恶性疟原虫基因组文库中分离出可能编码富含组氨酸蛋白质的DNA序列。测序显示，不同的DNA片段含有与探针高度同源的长重复区域。对一个克隆进行了全序列测定，发现它包含两个开放阅读框，这两个阅读框在重复区域重叠，但位于相反的链上。分析表明两者都在编码。一个阅读框可能编码一种小的富含组氨酸的蛋白质，另一个编码一种含有许多天冬氨酸残基的蛋白质。Southern印迹分析表明，这些序列在所研究的所有三种恶性疟原虫菌株中都是保守的。