de la Peña P, Zasloff M
Cell. 1987 Aug 14;50(4):613-9. doi: 10.1016/0092-8674(87)90034-1.
This report describes studies on the kinetics of herpes thymidine kinase (TK) mRNA transport in X. laevis oocytes as studied by microinjection and microdissection. We show that TK mRNA nuclear transport in this cell can be dramatically altered by introduction of specific DNA sequences into the nucleus. Introduction of DNA sequences encompassing the promoter results in enhancement, in trans, of a transport process that can deliver previously synthesized RNA to the cytoplasm. The report suggests that the promoter of a eukaryotic gene can functionally interact with the mechanism involved in mRNA nuclear transport.
本报告描述了通过显微注射和显微切割对非洲爪蟾卵母细胞中疱疹胸苷激酶（TK）mRNA转运动力学的研究。我们发现，通过向细胞核中引入特定DNA序列，该细胞中TK mRNA的核转运可发生显著改变。引入包含启动子的DNA序列会反式增强一种能将先前合成的RNA转运至细胞质的转运过程。该报告表明，真核基因的启动子可在功能上与mRNA核转运所涉及的机制相互作用。
