Koltunow A M, Gregg K, Rogers G E
Department of Biochemistry, University of Adelaide, SA, Australia.
Nucleic Acids Res. 1987 Oct 12;15(19):7795-807. doi: 10.1093/nar/15.19.7795.
Experiments concerning gene transcription in Xenopus oocytes have revealed that the efficiency of the HSV-TK promoter is dependent upon the nature of the attached gene sequences. Transcriptional efficiency of the TK promoter when attached to its own gene was 30-fold higher than that observed when the promoter was attached either to an avian keratin gene or to the chicken histone H2B gene. Furthermore, attachment of the keratin gene promoter to the TK gene resulted in a 20-fold increase in keratin promoter efficiency. It was found by subsequent experiments that the TK gene is able to exert a stimulatory influence upon attached promoter sequences. This cis effect was shown to be independent of orientation but dependent upon the distance between the DNA sequences involved.
关于非洲爪蟾卵母细胞基因转录的实验表明，单纯疱疹病毒胸苷激酶（HSV-TK）启动子的效率取决于所连接基因序列的性质。当TK启动子连接自身基因时，其转录效率比连接禽类角蛋白基因或鸡组蛋白H2B基因时高30倍。此外，角蛋白基因启动子与TK基因连接后，角蛋白启动子效率提高了20倍。后续实验发现，TK基因能够对所连接的启动子序列产生刺激作用。这种顺式效应与方向无关，但取决于相关DNA序列之间的距离。
