Matlashewski G, Schneider J, Banks L, Jones N, Murray A, Crawford L
EMBO J. 1987 Jun;6(6):1741-6. doi: 10.1002/j.1460-2075.1987.tb02426.x.
The close association of human papillomavirus type 16 DNA with a majority of cervical carcinomas implies some role for the virus in this type of cancer. To define the transforming properties of HPV-16 DNA in vitro we have now performed transfection experiments on baby rat kidney cells using HPV-16 DNA in conjunction with an activated ras gene. We have demonstrated that a 6.6-kb DNA fragment, containing the early genes of HPV-16 under the control of Moloney murine leukaemia virus long terminal repeats (MoMuLV-LTRs), cooperates with EJ-ras in transforming these cells. Both DNAs are required and neither alone is effective. The cooperating activity appears to reside in a protein or proteins derived from the E6/E7 region of the HPV-16 genome.
人乳头瘤病毒16型（HPV-16）DNA与大多数宫颈癌密切相关，这表明该病毒在这类癌症中发挥了某种作用。为了在体外确定HPV-16 DNA的转化特性，我们现在使用HPV-16 DNA与激活的ras基因相结合，对新生大鼠肾细胞进行了转染实验。我们已经证明，一个6.6 kb的DNA片段，包含在莫洛尼鼠白血病病毒长末端重复序列（MoMuLV-LTRs）控制下的HPV-16早期基因，与EJ-ras协同作用可转化这些细胞。两种DNA都是必需的，单独一种都无效。协同活性似乎存在于源自HPV-16基因组E6/E7区域的一种或多种蛋白质中。
