Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
Int J Pharm. 2019 Jan 10;554:93-104. doi: 10.1016/j.ijpharm.2018.10.064. Epub 2018 Oct 29.
The combination of photothermal therapy and targeted chemotherapy can produce much greater cytotoxicity than chemotherapy. Herein, we developed multifunctional targeted polymeric nanoparticles (NPs) loaded with indocyanine green (ICG) and doxorubicin (DOX) for the targeted photoacoustic imaging and photothermal ablation of oral cancer cells. The chemokine SDF-1, a specific antibody, was conjugated to NPs by the carbodiimide method. The NPs were automatically targeted to tumour tissue in vitro and in vivo through CXCR4-SDF-1 interactions. The results of in vivo and in vitro photoacoustic imaging and photothermal therapy experiments showed that the multifunctional NPs had excellent photoacoustic imaging characteristics and photothermal therapy capabilities. The photothermal material heated rapidly after laser irradiation, and the resulting heat increased cell metabolism and membrane permeability, which increased cellular NP uptake. The encapsulated drug (DOX) was released immediately after the liquid core was transformed into a gas via laser effects, which killed tumour cells while producing strong photoacoustic signals in vitro and in vivo. Thus, we concluded that the chemokine SDF-1 can be applied for the targeted chemotherapy of metastatic lymph nodes of oral squamous cell carcinoma (OSCC) and is more effective for treating oral cancer when combined with photothermal therapy than when used alone.
光热疗法和靶向化疗的联合使用可以产生比化疗更大的细胞毒性。在此，我们开发了负载吲哚菁绿（ICG）和阿霉素（DOX）的多功能靶向聚合物纳米粒子（NPs），用于口腔癌细胞的靶向光声成像和光热消融。趋化因子 SDF-1 是一种特异性抗体，通过碳二亚胺法将其连接到 NPs 上。NPs 通过 CXCR4-SDF-1 相互作用在体外和体内自动靶向肿瘤组织。体内和体外光声成像和光热治疗实验的结果表明，多功能 NPs 具有优异的光声成像特性和光热治疗能力。激光照射后，光热材料迅速升温，产生的热量增加细胞代谢和膜通透性，从而增加细胞对 NP 的摄取。包裹的药物（DOX）在激光作用下将液相转化为气相后立即释放，在体外和体内杀死肿瘤细胞的同时产生强烈的光声信号。因此，我们得出结论，趋化因子 SDF-1 可用于口腔鳞状细胞癌（OSCC）转移性淋巴结的靶向化疗，与光热疗法联合使用时比单独使用时更有效地治疗口腔癌。
