Baek Hyun Jin, Jeong Yong Joon, Kwon Jeong Eun, Ra Jong Sung, Lee Sung Ryul, Kang Se Chan
Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea.
Genencell Co., Ltd, Yongin 16950, Republic of Korea.
Evid Based Complement Alternat Med. 2018 Oct 2;2018:3468040. doi: 10.1155/2018/3468040. eCollection 2018.
The extract of the fruit (MCE) is recognized as an alternative treatment for diabetes. The extract of leaves (LFE) is traditionally used as a folk medicine for treating inflammatory diseases in Korea as well. In this study, we investigated the synergistic effect of MCE combined with LFE on antihyperglycemic and antihyperlipidemic potentials. Based on the -glucosidase inhibitory effect and promotion of adipocyte differentiation in the 3T3-L1 cell line, the MLM was prepared with MCE:LFE (8:2 weight:weight). MLM showed the synergistic effects in the promotion of the glucose uptake rate, suppression of dipeptidyl peptidase-4 (DPP-4) mRNA expression, upregulation of an insulin receptor substrate and glucose transporter type-4 expression, and an increase in insulin-associated signaling in C2C12 cells. In addition, the efficacy of peroxisome proliferator-activated receptor- agonism and glucose uptake rate by MLM supplementation was significantly enhanced . Then, the antihyperglycemic and antihyperlipidemic effects of MCE, LFE, and MLM at the dose of 50, 100, and 200 mg/kg/day (n = 6 per each group) were determined in streptozotocin (STZ)-insulted mice fed an atherogenic diet (ATH) for 4 weeks. In addition, MLM (50, 100, and 200 mg/kg/day, n = 5 per each group) was supplemented in ATH-fed mice for 10 weeks. Compared with MCE or LFE alone, MLM supplementation led to a more significant reduction of glucose levels in both STZ/ATH and /ATH mice as well as lowered lipid profiles in STZ/ATH mice. In addition, the stimulation of islet of Langerhans regeneration was more pronounced by MLM supplementation in both mice models. In conclusion, antihyperglycemic and antihyperlipidemic effects were strengthened by the combined extracts of and (MLM) in diabetes-mimicking mice.
该果实提取物（MCE）被认为是糖尿病的一种替代治疗方法。叶提取物（LFE）在韩国传统上也被用作治疗炎症性疾病的民间药物。在本研究中，我们研究了MCE与LFE联合使用对降血糖和降血脂潜力的协同作用。基于3T3-L1细胞系中的α-葡萄糖苷酶抑制作用和脂肪细胞分化促进作用，以MCE:LFE（8:2重量:重量）制备了MLM。MLM在促进葡萄糖摄取率、抑制二肽基肽酶-4（DPP-4）mRNA表达、上调胰岛素受体底物和葡萄糖转运蛋白4型表达以及增加C2C12细胞中胰岛素相关信号传导方面显示出协同作用。此外，通过补充MLM，过氧化物酶体增殖物激活受体激动作用和葡萄糖摄取率的功效显著增强。然后，在喂食致动脉粥样硬化饮食（ATH）4周的链脲佐菌素（STZ）损伤小鼠中，测定了MCE、LFE和MLM在50、100和200mg/kg/天剂量下（每组n = 6）的降血糖和降血脂作用。此外，在喂食ATH的小鼠中补充MLM（50、100和200mg/kg/天，每组n = 5）10周。与单独使用MCE或LFE相比，补充MLM导致STZ/ATH和ATH小鼠的血糖水平更显著降低，以及STZ/ATH小鼠的血脂水平降低。此外，在两种小鼠模型中，补充MLM对胰岛再生的刺激作用更明显。总之，在模拟糖尿病的小鼠中，果实和叶的联合提取物（MLM）增强了降血糖和降血脂作用。
