Leibniz Institute of Photonic Technology , Jena 07745 , Germany.
Friedrich Schiller University , Institute of Physical Chemistry , Jena 07743 , Germany.
Anal Chem. 2018 Nov 20;90(22):13243-13248. doi: 10.1021/acs.analchem.8b01355. Epub 2018 Nov 2.
Fiber-enhanced Raman spectroscopy was developed for the chemically selective and sensitive quantification of the important antibiotic cefuroxime in human urine. A novel optical sensor fiber was drawn and precisely prepared. In this fiber structure, light is strongly confined in the selectively filled liquid core, and the Raman scattered signal is collected with unprecedented efficiency over an extended interaction length. The filling, emptying, and robustness are highly improved due to the large core size (>30 μm). Broadband step-index guidance allows the free choice of the most suitable excitation wavelength in complex body fluids. The limit of detection of cefuroxime in human urine was improved by 2 orders of magnitude (to μM level). The quantification of cefuroxime was achieved in urine after oral administration. This method has great potential for the point-of-care monitoring of antibiotics concentrations and is an important step forward to enable clinicians to rapidly adjust doses.
纤维增强拉曼光谱法用于在人尿液中对重要抗生素头孢呋辛进行化学选择性和灵敏定量。新型光学传感器纤维被拉制并精确制备。在这种纤维结构中，光在选择性填充的液体芯中强烈限制，并且拉曼散射信号在扩展的相互作用长度上以前所未有的效率被收集。由于大芯径（>30 μm），填充、排空和稳健性得到了极大的提高。宽带阶跃指数引导允许在复杂体液中自由选择最合适的激发波长。头孢呋辛在人尿液中的检测限提高了两个数量级（达到 μM 水平）。在口服后，头孢呋辛在尿液中的定量得以实现。该方法对于即时检测抗生素浓度具有很大的潜力，并且是向前迈出的重要一步，使临床医生能够快速调整剂量。
